FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863037
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGAAGA26100.30242040607760734No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21870.2534074437133054No Hit
GTATATTACTGTGCGAGAGTAAACTGTATTAGTACCAGTTGCTGGGGGTA21790.252480484614217No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17070.19778989776799835No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14150.1639558906512699No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA14140.16384002076388382No Hit
GTATTAGTACCAGTTGCTGGGGGTACTTTGACTATTGGGGCCAGGGAACC13740.15920522526844155No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC13490.15630847808379014No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13300.15410695022345508No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12760.14784997630460803No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12070.13985495407497014No Hit
GCCGTATATTACTGTGCGAGAGTAAACTGTATTAGTACCAGTTGCTGGGG11990.13892799497588168No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11640.13487254891736972No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10710.12409664939046645No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAA10600.12282208062921983No Hit
ATATACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGAAGACTG10500.12166338175535928No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10310.1194618538950242No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9700.11239379076447475No Hit
GAGTTACAGTTCCCCTCGGACGTTCGGCCAGGGGACCAAGGTGGAAATCA9570.11088748222845603No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9540.11053987256629785No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT9330.10810660493119066No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9220.10683203616994405No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAGGGTTCAGTG9200.10660029639517193No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9070.1050939878591532No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8950.10370354921052052No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG8910.10324006966097629No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8870.10277659011143207No Hit
GTCTTCATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTATATTA8760.10150202135018546No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG8740.10127028157541335No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)