FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences905716
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGCCAGATTGAATGGTCCGGTGTTCGG24830.2741477460925941No Hit
GTAATAATCAGCCTCATCATCAGACTGGAGCCCACTGATGGCCAGGGAGG23110.25515724575915627No Hit
GTCTACTACTGTGCGAGAAGGTTAACTCCCAATAGACACGGCATGGACGT19780.21839075383453532No Hit
GTAGTAGACAGCCGTGTCGTCGACTCTCAGGTTGTTCATTTCCAGATAGA19690.21739706486359964No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17310.1911195120766333No Hit
CCTCACTCACTGTGCAGTTCCAGGGTCCTGGGCCCAGTCTGTGCTGACTC16820.1857094276793167No Hit
GCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGG16160.17842237522578822No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA13560.1497158049543124No Hit
GTGCTGACTCAGCCACACTCAGTGTCTGGGACCCCCGGGCAGAGGGTCAC13090.14452654032831486No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12390.13679784833214825No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT12010.13260227267708644No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC11040.12189251376811273No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA10710.11824898754134851No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10460.11548874039986044No Hit
TCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC10340.11416382177194617No Hit
GTTCCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACACTCAGTGTC10080.11129316474479858No Hit
GTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC10020.11063070543084144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC13050.061.168024
GTATCAA13850.060.80741
ATCAACG13700.058.2594453
CAACGCA13750.058.0636445
AACGCAG14400.055.445786
GGTATCA5350.049.7815321
TATCAAC16200.049.2687952
ACGCAGA17400.045.886167
CGCAGAG17450.045.7572178
GCAGAGT20900.037.859819
AGTACGG1407.0332135E-735.962845
TAGACAG11200.032.109685
GTACGGA1153.31174E-431.2737646
CAGCCGT8450.030.6462849
GAATACG1902.2239328E-730.2878469
TATTGAG12400.030.1623845
CTGTGCG9450.029.6868959
GTACATG25500.028.7925621
TAGTACG2003.493351E-728.7655014
AGTAGAC10450.028.2118453
Produced by FastQC (version 0.11.5)