FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129860
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTC32810.2903899598180305No Hit
GTAATATACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTGAA31920.28251287770166217No Hit
GTATATTACTGTGCGAAGTTGTATCACTATGATAGTAGTGGTCGTTTCTA27190.24064928398208627No Hit
GTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAAT24190.21409732179208044No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC21280.18834191846777476No Hit
CTACTATCATAGTGATACAACTTCGCACAGTAATATACGGCCGTGTCCTC18320.16214398244030234No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA18100.16019683854636857No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18010.1594002796806684No Hit
TGATAGTAGTGGTCGTTTCTACGACGATTACTGGGGCCAGGGAACCCTGG17730.1569220965429345No Hit
GACCTAGGACGGTCAGGGTGGTCCCTCCGCCGAACACTCTGTAGCTGCCT17450.15444391340520064No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17150.15178871718620007No Hit
CTTATAGACATCATAAATTATGAATTTGGGGGCTTTGCCTGGGTGCTGTT16630.1471863770732657No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15530.1374506576035969No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT15280.1352379940877631No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA15120.13382188943762943No Hit
CTATGATAGTAGTGGTCGTTTCTACGACGATTACTGGGGCCAGGGAACCC14960.1324057847874958No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14780.13081266705609543No Hit
GCCGTATATTACTGTGCGAAGTTGTATCACTATGATAGTAGTGGTCGTTT14320.12674136618696122No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14120.12497123537429417No Hit
CTCTGTAGCTGCCTGCATATGAGCAGCAGTAATAATCAGCCTCATCCTCA13780.12196201299276017No Hit
GCCTTGGGCTGACCTAGGACGGTCAGGGTGGTCCCTCCGCCGAACACTCT13570.12010337563945976No Hit
ATATACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTGAACTG13380.11842175136742604No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT13240.11718265979855912No Hit
GATTATTACTGCTGCTCATATGCAGGCAGCTACAGAGTGTTCGGCGGAGG12750.11284583930752483No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT12650.1119607739011913No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG12470.11036765616979094No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12230.10824349919459048No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11380.10072044324075549No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG11370.10063193670012213No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12350.071.126291
TCAACGC14000.059.6578034
ATCAACG14200.058.817553
TATCAAC15650.053.828072
CAACGCA16000.052.200585
AACGCAG16350.051.0763556
CAGCGCT5600.037.2811789
GTACATG28150.036.3201181
TACATGG29250.033.477292
ACATGGG29250.032.4926643
GGTATCA6500.032.1234321
GTATGTA11600.030.4141271
GCAGCGC7350.030.3636518
ACACGGG9850.029.9658957
GTACACG10100.029.9409285
GGCAGCG7400.029.1856387
TACACGG10350.028.5182676
TATGTAC10850.027.8712772
TATTGAG11950.027.7157885
TATACGG17350.026.9743615
Produced by FastQC (version 0.11.5)