FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443870
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCGCGGCGGTCACAGAGTTCAGGTTCAGGGAGA47911.079370085835943No Hit
GTCTATGGTGGGTCCTTCAGTGGTTACTACTGGAGCTGGATCCGCCAGCC45601.0273278212089125No Hit
GTATATTACTGTGCGAGAGGATCCGTAGGATATTGCAGTGATGGCAGCTG32100.7231847162457475No Hit
ATATACAGCCGTGTCCGCGGCGGTCACAGAGTTCAGGTTCAGGGAGAACT17270.38907788316398945No Hit
GCTGTATATTACTGTGCGAGAGGATCCGTAGGATATTGCAGTGATGGCAG13790.31067654944015144No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG11140.25097438439182645No Hit
ACCATAGACAGCGCAGGTGAGGGACAGGGTCTCCGAGGGCTTCAACAGTC10750.2421880280262239No Hit
CAGTAATATACAGCCGTGTCCGCGGCGGTCACAGAGTTCAGGTTCAGGGA10280.23159934214972852No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10070.22686822718363484No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTCATGTCTTCGG9720.21898303557347873No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9590.21605425011827786No Hit
ATATTACTGTGCGAGAGGATCCGTAGGATATTGCAGTGATGGCAGCTGCT9500.21402662941852343No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA9380.2113231351521842No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9280.2090702232635682No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9220.20771847613039854No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC8720.19645391668731838No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC8680.19555275193187194No Hit
ATCCTACGGATCCTCTCGCACAGTAATATACAGCCGTGTCCGCGGCGGTC8600.19375042242097912No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT8300.186991686755131No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG8260.1860905219996846No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT8250.18586523081082298No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8120.18293644535562215No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG7920.17843062157839007No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC7720.173924797801158No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA7650.17234775947912676No Hit
GTCCCAGGTGCAGCTAAAGCAGTGGGGCGCAGGACTGTTGAAGCCCTCGG6880.15500033793678328No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA6820.15364859080361368No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6740.15184626129272086No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6730.15162097010385922No Hit
GTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCAGTGGTTACTACTGGA6620.1491427670263816No Hit
TCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC6390.14396106968256472No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAGCAGACACGTCC6360.1432851961159799No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG6290.14170815779394869No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6080.136977042827855No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6060.13652646045013178No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCT6050.1363011692612702No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT5910.13314709261720772No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC5760.1297677247842837No Hit
GTTCAGGTTCAGGGAGAACTGGCTCTTGGACGTGTCTGCTGATATGGTGA5640.12706423051794444No Hit
GTTGAAGCCCTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGT5410.12188253317412755No Hit
GACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATGACCACTCAGG5380.12120665960754275No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCA5310.11962962128551152No Hit
GTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG5290.1191790389077883No Hit
CTGTAGCAGCCACAGAGGTCACGCTCAGGTGGTCCTTGAACAGCGCTCTT5240.11805258296348028No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG5060.11399734156397143No Hit
CTACTGGAGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTG5020.11309617680852502No Hit
ATACAGCCGTGTCCGCGGCGGTCACAGAGTTCAGGTTCAGGGAGAACTGG4980.11219501205307861No Hit
GTTCCATGGCTCGGCACAGCCCGGCAGGACACTGGACACGCTGTAGCAGC4960.1117444296753554No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT4910.11061797373104738No Hit
CTGCAATATCCTACGGATCCTCTCGCACAGTAATATACAGCCGTGTCCGC4880.10994210016446256No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT4880.10994210016446256No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGA4850.10926622659787776No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA4800.10813977065356974No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA4780.10768918827584653No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG4720.10633744114267692No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC4650.10476040282064569No Hit
CCGTAGGATATTGCAGTGATGGCAGCTGCTACGGCGTGGGGTTTGACTAC4640.1045351116317841No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTC4630.10430982044292247No Hit
GTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAG4570.10295807330975286No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT4530.10205690855430644No Hit
CGCTGTAGCAGCCACAGAGGTCACGCTCAGGTGGTCCTTGAACAGCGCTC4500.10138103498772164No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGCTGACCAGGGTTCCCTGG4490.10115574379886003No Hit
GTGCGAGAGGATCCGTAGGATATTGCAGTGATGGCAGCTGCTACGGCGTG4490.10115574379886003No Hit
TTCTGAGAGTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTC4490.10115574379886003No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATATAC16100.048.309823
GTAATAT16100.047.889491
TAATATA16800.045.8682332
ACAGCCG17100.045.4693078
TATACAG17600.045.4047245
CAGCCGT17250.045.073929
ATATACA18750.043.3927154
ATACAGC18500.043.1958436
TACAGCC18450.042.5324947
TACTGTG20350.042.452927
CTGTGCG13650.042.193749
TATTACT20450.041.5459144
GGTCTAC700.001088254941.1760061
TTACTGT20500.041.088736
ATTACTG20600.040.889275
GTATATT14400.040.532631
TATATTA14750.039.5485462
GTCTATA750.001527717338.430941
ATGGGAG4700.038.294195
ATATTAC15100.037.6779823
Produced by FastQC (version 0.11.5)