FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443870
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGGGTCCTTCAGTGGTTACTACTGGAGCTGGATCCGCCAGCC46021.0367900511411No Hit
GTAATATACAGCCGTGTCCGCGGCGGTCACAGAGTTCAGGTTCAGGGAGA45411.0230472886205422No Hit
GTATATTACTGTGCGAGAGGATCCGTAGGATATTGCAGTGATGGCAGCTG30170.6797035167954582No Hit
ATATACAGCCGTGTCCGCGGCGGTCACAGAGTTCAGGTTCAGGGAGAACT17390.3917813774303287No Hit
GCTGTATATTACTGTGCGAGAGGATCCGTAGGATATTGCAGTGATGGCAG12680.2856692274765134No Hit
ACCATAGACAGCGCAGGTGAGGGACAGGGTCTCCGAGGGCTTCAACAGTC10900.24556739585914794No Hit
CAGTAATATACAGCCGTGTCCGCGGCGGTCACAGAGTTCAGGTTCAGGGA9720.21898303557347873No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTCATGTCTTCGG9680.21808187081803232No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9630.21695541487372427No Hit
ATATTACTGTGCGAGAGGATCCGTAGGATATTGCAGTGATGGCAGCTGCT9250.20839434969698334No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8720.19645391668731838No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT8580.1932998400432559No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA8530.19217338409894788No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA8500.19149751053236308No Hit
ATCCTACGGATCCTCTCGCACAGTAATATACAGCCGTGTCCGCGGCGGTC8110.18271115416676054No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8110.18271115416676054No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC7840.17662829206749722No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC7780.17527654493432762No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG7700.17347421542343477No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7670.17279834185684997No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7530.16964426521278753No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT7250.16333611192466263No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA7110.16018203528060018No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG6840.15409917318133687No Hit
GTCCCAGGTGCAGCTAAAGCAGTGGGGCGCAGGACTGTTGAAGCCCTCGG6710.15117038772613603No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6670.15026922297068962No Hit
GTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCAGTGGTTACTACTGGA6550.1475657287043504No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC6470.14576339919345754No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCT6230.14035641066077906No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG6190.13945524590533265No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG6140.13832878996102463No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC5980.13472413093923896No Hit
TCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC5850.13179534548403812No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGGCACCCTCAGCGTCT5850.13179534548403812No Hit
GTTCAGGTTCAGGGAGAACTGGCTCTTGGACGTGTCTGCTGATATGGTGA5690.12819068646225246No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA5690.12819068646225246No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGGCACCCTCAGCGTCTGGGACCC5630.12683893932908286No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC5540.12481131862932841No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCA5500.12391015387388199No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGA5470.12323428030729719No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG5430.12233311555185077No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAGCAGACACGTCC5430.12233311555185077No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA5390.12143195079640436No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG5320.11985491247437313No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTC5250.11827787415234191No Hit
GTTGAAGCCCTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGT5210.11737670939689548No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC5150.11602496226372587No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT5080.11444792394169465No Hit
GGTTACTACTGGAGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTG5020.11309617680852502No Hit
GTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCG5000.1126455944308018No Hit
CTGCAATATCCTACGGATCCTCTCGCACAGTAATATACAGCCGTGTCCGC5000.1126455944308018No Hit
ATACAGCCGTGTCCGCGGCGGTCACAGAGTTCAGGTTCAGGGAGAACTGG4940.11129384729763218No Hit
CCCTCATGCTGCCACCCCCGACTGTCACTGCACCGACCGGCCCTCGAGGA4930.11106855610877059No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT4900.11039268254218577No Hit
GACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATGACCACTCAGG4850.10926622659787776No Hit
GTTCCATGGCTCGGCACAGCCCGGCAGGACACTGGACACGCTGTAGCAGC4850.10926622659787776No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT4820.10859035303129294No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGCTGACCAGGGTTCCCTGG4800.10813977065356974No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG4780.10768918827584653No Hit
CTACTGGAGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTG4670.1052109851983689No Hit
CTGTAGCAGCCACAGAGGTCACGCTCAGGTGGTCCTTGAACAGCGCTCTT4640.1045351116317841No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA4610.10385923806519927No Hit
CCGTAGGATATTGCAGTGATGGCAGCTGCTACGGCGTGGGGTTTGACTAC4570.10295807330975286No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA4480.10093045260999843No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC4460.10047987023227521No Hit
GTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG4450.10025457904341362No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCGT100.007238477143.995159
TATAACG300.00198648572.021932
CCGTCCG350.00366477361.7122089
ACTACCG350.00366477361.7122088
GAATACG1600.049.4983339
TATACCG450.00989077148.014625
ATACCGT450.00989958648.0037966
CTGTGCG11700.046.7676549
GTGCTAG1700.046.602431
TGGACCG952.0078587E-645.4875375
TCTATGG16250.043.877982
CTATGGT16050.043.5272753
GTCTATG16300.043.301531
GTGGGTC16250.042.9770168
GTATATT13250.042.941381
TATATTA13700.041.0051882
ACAGCCG15950.040.17428
AATATAC16700.039.676753
ATATTAC14050.039.4710963
CAGCCGT16100.039.352719
Produced by FastQC (version 0.11.5)