FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808115
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCGTCAGATCTCAGGCTTCTCAGCTCCATGTAGG32280.3994480983523385No Hit
GTCTATTACTGTGCGATAAGCTCTGACGTGACGATTTTTCAAGTGGGCCG20050.24810825191959066No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT14970.18524591178235772No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA14160.17522258589433434No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC13790.1706440296244965No Hit
CCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG13030.16123942755672152No Hit
GTCTGGAACTGTGGTGGAGAGGCCTGGGGCCTCAGTGAGGGTCTCCTGCA12520.15492844459018829No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12200.1509686121405988No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAAGTGGTACGT11090.1372329433310853No Hit
GTACAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10050.12436348786991949No Hit
GCCTACATGGAGCTGAGAAGCCTGAGATCTGACGACACGGCCGTCTATTA9760.12077488971247904No Hit
GTGGTGGAGAGGCCTGGGGCCTCAGTGAGGGTCTCCTGCAAGCCTTCTGC9450.11693880202693924No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8920.11038032953230666No Hit
GGATGGAACAGCGCTTACAATGGTGACACAAACTATGCACAGAAGTTCCA8560.1059255180265185No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8420.1041930913298231No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA8160.10097572746453165No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)