FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences803063
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT22090.27507181877386955No Hit
GAAATAGACGGCCGTGTCGTCAGATGTCAGGCTCCCAAGCGCCATGTAGA15900.19799193836598122No Hit
TCTATGATGCGTCCAGTAGGGCCGCTGGCATCCCAGACAGGTTCAGTGGC15560.19375814848897285No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15270.19014697477034803No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA13940.17358538495734457No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG13790.17171753648219382No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13370.16648756075177165No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13320.16586494459338808No Hit
GTAGAGAGTAGTCGTGGAATCGTCCGCGGTAATCGCGACTCTGCCCTGGA13130.1634990031915304No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC12890.1605104456312892No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA12700.15814450422943158No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCAGTGAAGGTCTCTTGTA12630.15727284160769456No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12520.15590308605925063No Hit
GTCTCCAGCCACCCTGTCTCTGTCTCCAGGGGAAAGAGCCACCCTCTCCT12240.15241643557230256No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC11820.1471864598418804No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT11770.14656384368349681No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11730.14606575075678993No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11620.14469599520834606No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC11430.14233005380648842No Hit
CTATTACTGTCAGCAGTATGATAGGCCACCATTCAACTTCGGCCCTGGGA11170.13909244978289376No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG10820.13473413667420864No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10480.1305003467972002No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA10300.12825892862701932No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC10110.12589298722516165No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10090.12564394076180824No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9920.12352704582330401No Hit
ACTCTCTACATGGCGCTTGGGAGCCTGACATCTGACGACACGGCCGTCTA9900.12327799935995058No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9740.1212856276531231No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9590.11941777917797235No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTGAT9450.1176744539344983No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9340.11630469838605438No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9300.11580660545934753No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9130.11368971052084331No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9060.11281804789910631No Hit
ATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGA9000.112070908509046No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG8690.10821068832706773No Hit
GTATGATAGGCCACCATTCAACTTCGGCCCTGGGACCAAAGTGGATATCA8620.1073390257053307No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC8600.10708997924197727No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAACACCTACTTAGCCTGGTACC8500.1058447469252101No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8460.10534665399850322No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTGATCACCATGGACTGGACCTG8280.10310523582832232No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT8130.10123738735317155No Hit
GTCTATTACTGTCAGCAGTATGATAGGCCACCATTCAACTTCGGCCCTGG8130.10123738735317155No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT8120.10111286412149484No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA8100.10086381765814138No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)