FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences817476
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG17520.2143182185165069No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGA16120.19719233347523352No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG15230.18630516369899544No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14960.18300231444103557No Hit
GTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTA14640.17908782643160168No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC13140.1607386638873802No Hit
GCTATAGGCACAGTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCA12940.15829210888148398No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT12380.15144175486497463No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA12000.14679330035377186No Hit
ACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG11270.13786337458225073No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10740.13138000381662582No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT10550.1290557765610244No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG10330.1263645660545386No Hit
CATGTAGACTGTGCTCGTGGACGTGTCCCTGGTCATGGTGACTCTGCCCT10030.1226947335456943No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCACTCAGGCTG9780.11963653978832407No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAAC9770.11951421203802925No Hit
CCATGTAGACTGTGCTCGTGGACGTGTCCCTGGTCATGGTGACTCTGCCC9510.1163336905303642No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG9480.11596670727947976No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG8890.10874937001208598No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT8850.10826005901090675No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCC8730.10679212600736904No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT8670.10605815950560016No Hit
GCCTATAGCAGTGGCTGGTACGGGGACTACTACTACTACGGTATGGACGT8350.10214367149616625No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC8330.10189901599557663No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15350.063.822651
ATCAACG17150.055.8400543
CAACGCA17500.054.7199025
TCAACGC17400.054.6239364
GGTATCA6750.053.3593521
AACGCAG18450.051.902356
TATCAAC18650.051.3488962
ACGCAGA20550.046.9459537
CGCAGAG22550.042.7822348
GGTATAT2507.6397555E-1131.6954561
ATAGGTG9400.030.6401214
ATAGGCG950.004888098730.3175933
GTACATG19600.029.769621
CCCCGTT4250.028.7981877
CCGTTGA4500.028.7981879
CAGAGTA24650.028.6229469
TAGGTGG10050.027.9402525
ACATGGG20150.027.8726253
CCCGTTG4400.027.8164338
CAGAGCG8800.027.8164339
Produced by FastQC (version 0.11.5)