FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715529
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATAAATAGCCGTGTCCTCAAGTCCCAGTCTGTTCATTTGCAGATATA17660.2468104018146015No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15670.21899881067014754No Hit
ATTTATTATTGTGCGAGTGACTGGAACTACGGCTACTGGGGCCAGGGTAC15610.21816027023363135No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA14660.2048833799887915No Hit
TTTAAGAGGTGTCCAGTGTCAGGGGCAGCTGGTGGAGTCTGGGGGAGGCG14510.202787028897501No Hit
GATTTATAGTACAAGGCACACACACTCGTGGACCCCTGCCCGGTTCTCCG13080.18280181516053157No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC12840.17944765341446678No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12180.17022370861278857No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11680.16323587164182024No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11560.16155879076878785No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11530.16113952055052974No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10770.15051800835465787No Hit
CCATAGTAGAGTAGGCAGTAATACTCAGCCTCGTCCTCAGCCTGCGCACC10360.14478798203846385No Hit
ATATTATGCTGCCTCCGTGGAGGGCCGATTCACCATCTCCAGAGACAATG10330.14436871182020575No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC10160.1419928472500765No Hit
CTCCAGAGACAATGCCAAGAATACTCTATATCTGCAAATGAACAGACTGG9590.1340267131031726No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9340.13053279461768844No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT9220.12885571374465604No Hit
CTATAAATCAGTGCCCTGGGTGCTTGTCCAGGTTTCTGCTGGAACCAGTG8980.12550155199859125No Hit
TCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC8880.12410398460439757No Hit
CTACTATGGTGGTACTGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCT8870.12396422786497822No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAATCAC8800.12298593068904265No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8590.12005103916123597No Hit
CTCGTGGACCCCTGCCCGGTTCTCCGGCTCCCTCCTTGGGGGCAAAGCTG8490.11865347176704229No Hit
GGGTACCCTGGCCCCAGTAGCCGTAGTTCCAGTCACTCGCACAATAATAA8490.11865347176704229No Hit
GCATAATATTTTGTGTCTTCTACATATGAGATAACTGCCACCCACTCCAG8470.11837395828820355No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8170.11418125610562255No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG8130.11362222914794509No Hit
CTCTATATCTGCAAATGAACAGACTGGGACTTGAGGACACGGCTATTTAT8050.11250417523259015No Hit
GCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAC7880.11012831066246093No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA7810.10915001348652535No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT7730.10803195957117043No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA7540.10537658152220246No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT7540.10537658152220246No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG7520.10509706804336372No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC7510.10495731130394435No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG7460.1042585276068475No Hit
GACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACAGTACCACCA7430.10383925738858943No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA7370.10300071695207322No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)