FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715529
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17050.2382852407100201No Hit
ATAATAAATAGCCGTGTCCTCAAGTCCCAGTCTGTTCATTTGCAGATATA16640.2325552143938261No Hit
ATTTATTATTGTGCGAGTGACTGGAACTACGGCTACTGGGGCCAGGGTAC15840.22137467524027676No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA14230.19887384019375873No Hit
TTTAAGAGGTGTCCAGTGTCAGGGGCAGCTGGTGGAGTCTGGGGGAGGCG14120.19733651606014568No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC13100.18308132863937032No Hit
GATTTATAGTACAAGGCACACACACTCGTGGACCCCTGCCCGGTTCTCCG12610.17623324840782134No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12370.17287908666175655No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11210.15666730488911002No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11090.15499022401607762No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10810.15107703531233535No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10730.1499589813969804No Hit
ATATTATGCTGCCTCCGTGGAGGGCCGATTCACCATCTCCAGAGACAATG10380.14506749551730258No Hit
CCATAGTAGAGTAGGCAGTAATACTCAGCCTCGTCCTCAGCCTGCGCACC10140.1417133337712378No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10060.14059527985588285No Hit
CTACTATGGTGGTACTGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCT9640.13472549680026946No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC9580.13388695636375325No Hit
CTCCAGAGACAATGCCAAGAATACTCTATATCTGCAAATGAACAGACTGG9460.13220987549072083No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT9430.13179060527246275No Hit
CTCGTGGACCCCTGCCCGGTTCTCCGGCTCCCTCCTTGGGGGCAAAGCTG9420.1316508485330434No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9240.1291352272234948No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAATCAC9220.12885571374465604No Hit
CTATAAATCAGTGCCCTGGGTGCTTGTCCAGGTTTCTGCTGGAACCAGTG9040.12634009243510744No Hit
TCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC9000.12578106547742998No Hit
GCATAATATTTTGTGTCTTCTACATATGAGATAACTGCCACCCACTCCAG8860.12382447112555886No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8820.12326544416788139No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8700.121588363294849No Hit
GGGTACCCTGGCCCCAGTAGCCGTAGTTCCAGTCACTCGCACAATAATAA8470.11837395828820355No Hit
GCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAC8360.11683663415459052No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8140.11376198588736446No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG8020.11208490501433206No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA7620.10649463543755738No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT7590.10607536521929929No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG7410.10355974390975069No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG7310.10216217651555703No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGCATAGCTGC7290.1018826630367183No Hit
CTCTATATCTGCAAATGAACAGACTGGGACTTGAGGACACGGCTATTTAT7160.10006582542426654No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)