FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426686
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGACTGGCCTCCGGTCACTTTCGGCGGAGGGACCAGGGTGGAGA13490.3161575491110559No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT13290.3114702615037756No Hit
GTGTAGCAGCACCTCCTGCCTATTACCCATTGATGGTTTTGATTTCTGGG13280.3112358971234116No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10650.2495980650876757No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10460.24514514186075942No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCACCTGGCGGAGTCTGGGGGAGGCG10020.23483310912474278No Hit
GTATTATGGAGACTCCGTGAGGGGCCGATTCACCGTCTCCAGAGACAACT9560.2240523476279981No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA9520.22311489010654204No Hit
GTGTACAGGCCGTTCAATTGCAGATACACCATGTTCTTGGAGTTGTCTCT9380.21983378878144585No Hit
CTGCTACACCGATCTCTCGCACAGAAATAGACAGCCGTGTCCCCAGTGTA9290.2177245093581697No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8950.20975612042579322No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCCGGGCAAAGAGCCATCCTCTCCT8890.2083499341436091No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC8850.20741247662215306No Hit
TCATTATACTGCTGACAGTAATAAACTGCAACATCTTCAGACTGCAGGCT8540.2001471808308686No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC8510.19944408768977653No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8390.1966317151254084No Hit
CCATAATACTTATTTATTCCATCATAGGCTATAAATGCCATCCACTCCAG8340.1954598932235883No Hit
GTCTGGGACAGACTACACTCTCAGCATCAGCAGCCTGCAGTCTGAAGATG8160.19124133437703605No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC7990.1872571399108478No Hit
GTCCCAGACCCACTGCCACTGAACCTGACTGGGATACCAGTGGCCCTGGT7820.18327294544465952No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA7760.18186675916247547No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7510.1760076496533751No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC7500.17577328527301106No Hit
GACTACACTCTCAGCATCAGCAGCCTGCAGTCTGAAGATGTTGCAGTTTA7210.16897671824245464No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT7070.16569561691735843No Hit
GAAATAGACAGCCGTGTCCCCAGTGTACAGGCCGTTCAATTGCAGATACA6970.1633519731137183No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6830.16007087178862206No Hit
GCCTGTACACTGGGGACACGGCTGTCTATTTCTGTGCGAGAGATCGGTGT6750.15819595674570996No Hit
GTCATTATACTGCTGACAGTAATAAACTGCAACATCTTCAGACTGCAGGC6300.1476495596293293No Hit
GTCTATTTCTGTGCGAGAGATCGGTGTAGCAGCACCTCCTGCCTATTACC6260.14671210210787322No Hit
GTCTGTGTCTCCCGGGCAAAGAGCCATCCTCTCCTGCAGGGCCAGTCAGA6040.14155608573986492No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6000.14061862821840887No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG5960.1396811706969528No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5870.13757189127367667No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG5860.13733752689331263No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG5730.13429078994858046No Hit
GGGTGGAGATCCAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG5610.1314784173842123No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC5600.13124405300384828No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT5500.12890040920020812No Hit
GTACAGGCCGTTCAATTGCAGATACACCATGTTCTTGGAGTTGTCTCTGG5330.12491621473401987No Hit
TTATTACTGTCAGCAGTATAATGACTGGCCTCCGGTCACTTTCGGCGGAG5240.12280693531074374No Hit
GTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTG5200.12186947778928768No Hit
GCCTATGATGGAATAAATAAGTATTATGGAGACTCCGTGAGGGGCCGATT5070.11882274084455548No Hit
CTCCCAGACTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCA5040.11811964770346345No Hit
TCTCCACCCTGGTCCCTCCGCCGAAAGTGACCGGAGGCCAGTCATTATAC4950.11601036828018729No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT4890.11460418199800322No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT4850.11366672447654716No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAACTTTGGCATGCACTGGG4820.11296363133545512No Hit
ATCATAGGCTATAAATGCCATCCACTCCAGCCCCTTGCCTGGAGCCTGGC4800.11249490257472708No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG4790.11226053819436306No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC4730.11085435191217899No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG4710.11038562315145095No Hit
CTGGTATCCCAGTCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTACACT4680.1096825300103589No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4540.1064014286852627No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA4510.10569833554417066No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT4500.10546397116380664No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA4450.10429214926198657No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT4450.10429214926198657No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG4430.10382342050125853No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA4400.10312032736016648No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC4330.1014797766976184No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG4320.10124541231725437No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGTCAGGTTCAGTG4320.10124541231725437No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA4310.10101104793689035No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4310.10101104793689035No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC4280.10030795479579831No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGT4280.10030795479579831No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)