FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426686
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGACTGGCCTCCGGTCACTTTCGGCGGAGGGACCAGGGTGGAGA14390.3372503433438172No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT13780.32295411614161235No Hit
GTGTAGCAGCACCTCCTGCCTATTACCCATTGATGGTTTTGATTTCTGGG12580.2948303904979306No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12500.29295547545501843No Hit
GTATTATGGAGACTCCGTGAGGGGCCGATTCACCGTCTCCAGAGACAACT10090.23647365978729087No Hit
GTGTACAGGCCGTTCAATTGCAGATACACCATGTTCTTGGAGTTGTCTCT9700.22733344895309432No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA9500.222646161345814No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9420.2207712463029019No Hit
CTGCTACACCGATCTCTCGCACAGAAATAGACAGCCGTGTCCCCAGTGTA9330.21866196687962577No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCACCTGGCGGAGTCTGGGGGAGGCG9330.21866196687962577No Hit
CCATAATACTTATTTATTCCATCATAGGCTATAAATGCCATCCACTCCAG8870.2078812053828811No Hit
TCATTATACTGCTGACAGTAATAAACTGCAACATCTTCAGACTGCAGGCT8860.20764684100251707No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8260.1935849781806762No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC8240.19311624941994815No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC8210.1924131562788561No Hit
GTCCCAGACCCACTGCCACTGAACCTGACTGGGATACCAGTGGCCCTGGT8080.18936641933412393No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCCGGGCAAAGAGCCATCCTCTCCT8070.18913205495375993No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA8010.1877258686715758No Hit
GTCTGGGACAGACTACACTCTCAGCATCAGCAGCCTGCAGTCTGAAGATG8010.1877258686715758No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7580.17764820031592318No Hit
GACTACACTCTCAGCATCAGCAGCCTGCAGTCTGAAGATGTTGCAGTTTA7550.17694510717483114No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC7520.1762420140337391No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC7220.16921108262281864No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG6910.16194578683153418No Hit
GCCTGTACACTGGGGACACGGCTGTCTATTTCTGTGCGAGAGATCGGTGT6840.1603052361689861No Hit
GTCTGTGTCTCCCGGGCAAAGAGCCATCCTCTCCTGCAGGGCCAGTCAGA6810.15960214302789405No Hit
GAAATAGACAGCCGTGTCCCCAGTGTACAGGCCGTTCAATTGCAGATACA6790.159133414267166No Hit
GTCATTATACTGCTGACAGTAATAAACTGCAACATCTTCAGACTGCAGGC6670.15632104170279784No Hit
GTCTATTTCTGTGCGAGAGATCGGTGTAGCAGCACCTCCTGCCTATTACC6540.15327430475806564No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6490.15210248285624559No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6490.15210248285624559No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG6390.14975883905260542No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT6180.14483718706496113No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6070.14225917888095696No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT5960.1396811706969528No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT5910.13850934879513271No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG5890.1380406200344047No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA5670.13288460366639637No Hit
CTCCCAGACTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCA5620.1317127817645763No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT5620.1317127817645763No Hit
GTACAGGCCGTTCAATTGCAGATACACCATGTTCTTGGAGTTGTCTCTGG5440.12749422291802404No Hit
TCTCCACCCTGGTCCCTCCGCCGAAAGTGACCGGAGGCCAGTCATTATAC5430.12725985853766No Hit
GGGTGGAGATCCAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG5320.12468185035365587No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG5290.12397875721256381No Hit
GCCTATGATGGAATAAATAAGTATTATGGAGACTCCGTGAGGGGCCGATT5080.11905710522491951No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT5010.11741655456237139No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG4750.111323080672907No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAACTTTGGCATGCACTGGG4690.10991689439072293No Hit
TTATTACTGTCAGCAGTATAATGACTGGCCTCCGGTCACTTTCGGCGGAG4690.10991689439072293No Hit
GTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTG4650.10897943686926687No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA4610.1080419793478108No Hit
GTAATAAACTGCAACATCTTCAGACTGCAGGCTGCTGATGCTGAGAGTGT4600.10780761496744677No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC4530.1061670643048987No Hit
ATCATAGGCTATAAATGCCATCCACTCCAGCCCCTTGCCTGGAGCCTGGC4510.10569833554417066No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC4500.10546397116380664No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT4500.10546397116380664No Hit
CTGGTATCCCAGTCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTACACT4480.1049952424030786No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT4480.1049952424030786No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG4440.10405778488162254No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4350.10194850545834641No Hit
GAGTGTAGTCTGTCCCAGACCCACTGCCACTGAACCTGACTGGGATACCA4350.10194850545834641No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT4340.1017141410779824No Hit
TCTTCAGACTGCAGGCTGCTGATGCTGAGAGTGTAGTCTGTCCCAGACCC4340.1017141410779824No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG4320.10124541231725437No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGTCAGGTTCAGTG4300.10077668355652634No Hit
GCCTATTACCCATTGATGGTTTTGATTTCTGGGGCCAAGGGACTTGGGTC4290.10054231917616234No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC4270.1000735904154343No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAGCG2550.050.8206757
ATAAACG450.00990002948.0029344
CAGCGCT2550.047.9973039
ACGCTTG605.1148375E-447.9973038
GCAGCGC2700.045.3307888
GGAGCTC3350.040.833538
CCTATAC907.783596E-540.0024453
TACACGG4600.039.128246
TTACACG750.001533248838.4023444
GTACACG4500.036.8022465
TACATGG12850.036.422462
GTACATG12950.036.1454431
GTATAGG1001.4463444E-436.006421
CCTTATA1001.4473425E-436.00222
TTAGACA800.00210499536.0021974
CGCTTGC800.00210620735.997989
GAGCTCT3650.035.5048569
GTATTAG1251.32309815E-534.5661621
GGGAGCT4200.034.283797
ACATGGG13750.034.038443
Produced by FastQC (version 0.11.5)