FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144464
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCGCGGACCGGAGCCCACTGATGGCCAGGGAGG35410.3094024801129612No Hit
GTACTACAGGGGGCCCTGTAGAATTTTTGGAGTGGTTACCGGCTGACTAC34570.3020627997036167No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC24250.21188958324595616No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24120.2107536803254624No Hit
CCCTTAGACTCTCCTGTTCAGCCTCTGGATTCACTTTCAACACCGCCTGG24040.21005466314362006No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA22630.197734485313649No Hit
GATTATTACTGTGCGGCCTGGGATGGCAACCTGAGTGGTCCGATATTCGG21930.1916180849725286No Hit
CTGTAGTACAGTAATAAAAGGCTGTGTCCTCGTTTTTCAGGCTGTTCATT21860.1910064449384166No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA19970.17449216401739154No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC19300.16863789511946203No Hit
GCCTTTTATTACTGTACTACAGGGGGCCCTGTAGAATTTTTGGAGTGGTT18980.16584182639209272No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC18920.16531756350571097No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18720.16357002055110514No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18460.16129821471011757No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC17030.14880328258468592No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCGGACCACTCAGG15660.13683261334563604No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15580.1361335961637937No Hit
GATCAGGACTGAACACTGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA15330.13394916747053642No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCT13970.12206587537921681No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13920.12162898964056537No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCGG13760.1202309552768807No Hit
GTAGTACAGTAATAAAAGGCTGTGTCCTCGTTTTTCAGGCTGTTCATTTG13760.1202309552768807No Hit
GTCCTGGGCCCGGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC13730.11996882383368984No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG13380.11691062366312964No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG13300.11621160648128731No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT13290.11612422933355702No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13080.1142893092312209No Hit
GTGCAGGGTCCTGGGCCCGGTCTGTGCTGACTCAGCCACCCTCAGCGTCT12940.11306602916299684No Hit
GTCTAAGGGACCCCCCAGGCTTTACCAAGCCTCCCCCAGACTCCACCAGC12940.11306602916299684No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC12840.11219225768569392No Hit
TTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAACTGCTGATACCA12740.11131848620839101No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA12700.11096897761746984No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12490.10913405751513372No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA12480.10904668036740342No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12110.10581372590138265No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT11840.10345454291266479No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT11840.10345454291266479No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT11790.10301765717401334No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG11650.10179437710578926No Hit
ATAAAAGGCTGTGTCCTCGTTTTTCAGGCTGTTCATTTGCAGATATAGTA11550.10092060562848634No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)