FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144464
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTACAGGGGGCCCTGTAGAATTTTTGGAGTGGTTACCGGCTGACTAC34280.29952886241943827No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26610.2325105901103049No Hit
CCCTTAGACTCTCCTGTTCAGCCTCTGGATTCACTTTCAACACCGCCTGG25150.2197535265416824No Hit
GATTATTACTGTGCGGCCTGGGATGGCAACCTGAGTGGTCCGATATTCGG23910.20891876022312628No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC22720.19852087964322163No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA21810.19056955919976512No Hit
CTGTAGTACAGTAATAAAAGGCTGTGTCCTCGTTTTTCAGGCTGTTCATT20290.17728823274476085No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20230.1767639698583791No Hit
GTAATAATCAGCCTCATCCGCGGACCGGAGCCCACTGATGGCCAGGGAGG19370.16924953515357408No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC18600.16252149477834166No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC18560.16217198618742049No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC18010.15736624306225447No Hit
GCCTTTTATTACTGTACTACAGGGGGCCCTGTAGAATTTTTGGAGTGGTT17870.15614296299403038No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17230.15055082553929175No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16380.14312376798221702No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT16120.14085196214122941No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA15920.1391044191866236No Hit
GTCCTGGGCCCGGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC15270.13342490458415468No Hit
GTGCAGGGTCCTGGGCCCGGTCTGTGCTGACTCAGCCACCCTCAGCGTCT15250.13325015028869408No Hit
GATCAGGACTGAACACTGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA14960.13071621300451564No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14390.12573571558388905No Hit
GTCTAAGGGACCCCCCAGGCTTTACCAAGCCTCCCCCAGACTCCACCAGC14010.12241538397013797No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCT13990.1222406296746774No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT13890.1213668581973745No Hit
GTAGTACAGTAATAAAAGGCTGTGTCCTCGTTTTTCAGGCTGTTCATTTG13580.11865816661773547No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCGGACCACTCAGG13530.11822128087908401No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT13480.11778439514043255No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC13240.11568734359490557No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT13130.11472619496987237No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG13100.11446406352668148No Hit
GTATACTGGTATCAGCAGTTCCCAGGAACGGCCCCCAAACTCCTCATCTA12980.113415537753918No Hit
TTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAACTGCTGATACCA12430.10860979462875198No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGGGGGTCAC12420.10852241748102168No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12090.10563897160592209No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT12080.10555159445819179No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA12050.10528946301500092No Hit
GTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG11810.10319241146947392No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCGG11600.10135749136713781No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC11590.1012701142194075No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG11590.1012701142194075No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11490.10039634274210461No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCGGTCTGTGCTGACTCAGCCAC11490.10039634274210461No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATACG2451.8189894E-1235.26239
GTATCAA21750.035.1096761
TCAACGC22150.033.4896664
CAACGCA22550.032.8956155
ATCAACG22650.032.7546773
AACGCAG23050.031.8584546
TATCAAC23650.031.6784172
CGCAGAG23700.031.2884718
GGCAGCG7000.029.8260277
GCAGCGC7500.029.7574628
CAGCGCT7100.029.4059419
GTACATG35150.028.2835581
ACATGGG34750.028.1895663
TACATGG35250.027.793362
TCTACAC7650.027.3049163
GCAGAGT27950.026.5308329
CATGGGG13450.026.237424
GTATATA4400.024.5594641
GTGCTAG3851.8189894E-1124.3255621
CTGTGCG17650.024.0659379
Produced by FastQC (version 0.11.5)