FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672264
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGGAGATACA18160.27013197196339533No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT14320.21301155498435137No Hit
GTTTATTACTGTGCGAATATAGGATATTGTACTGGTGGTGTGTGCTCTGA12640.18802137255601964No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12090.17984006283245868No Hit
ATATTACTGTCAGCAGTATGCCACTCTCCCTCCGTGGACGTTCGGCCAAG10790.16050242166767817No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG9990.14860233479704402No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9310.138487260957005No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8090.12033962847928789No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7700.11453833612985376No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7560.11245582092749278No Hit
GTACAATATCCTATATTCGCACAGTAATAAACAGCCGTGTCCTCAGCTCT7470.11111706115454643No Hit
CTATTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT7450.11081955898278056No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7190.10695203074982448No Hit
CCGTAATAGTGGTAGGGGGAGTGGTGGTCAGAGCACACACCACCAGTACA6880.10234074708745373No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6840.10174574274392204No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG6830.1015969916580391No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA6800.10115073840039031No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11900.078.062881
TCAACGC13300.069.299134
ATCAACG13550.067.489143
CAACGCA14450.063.2856675
TATCAAC14750.061.99852
AACGCAG14900.061.374366
CGCAGAG16850.054.2636158
TATACGT450.00990154748.0040933
GCAGAGT19650.046.8977859
GAATACG1051.9306973E-434.2835359
ACGCAGA27600.033.1283267
GTGCTAG1751.0700387E-732.919541
GTACACG3950.030.9899835
TACATGG17100.030.3183732
GTACATG16650.030.2751031
CAGCCGT11950.030.1236119
ACATGGG16850.029.4861933
TAAACAG14250.028.297155
GTAATAA14200.027.3846511
TACACGG4550.026.9033956
Produced by FastQC (version 0.11.5)