FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1967406
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC49900.25363346457213204No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT46370.23569105715851227No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTCCTGACGCAGCCACCCTCAGCGTCT45440.2309640206444425No Hit
GTCCTGGGCCCAGTCTGTCCTGACGCAGCCACCCTCAGCGTCTGGGACCC43480.22100166412016634No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC42750.21729119459836962No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT42620.2166304260533921No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA36150.18374448385335818No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGACGCAGCCAC35140.17861082054237915No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA33870.17215562014144511No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA33740.17149485159646763No Hit
GTCCTGACGCAGCCACCCTCAGCGTCTGGGACCCCCGGACAGAGGGTCAC33620.1708849114011038No Hit
ACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAACCTTTGACAC32630.16585290478935208No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC32320.1642772259513288No Hit
GACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAACCTTTGACA31030.1577203688511675No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC30960.15736457040387192No Hit
GCATGGGATGACCGTGTCAAAGGTTTTGTCTTCGGAACTGGGACCACGGT30780.15644966011082614No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC30670.15589054826507595No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG29830.15162096689752902No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT29810.15151931019830173No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28420.14445416960200386No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA27940.14201440882054847No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27660.14059121503136618No Hit
ACTGTAAACTGGTACCAGCAACTCCCAGGAGCGGCCCCCAAACTCCTCAT26990.13718571560725137No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG26100.1326619924916362No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25960.13195039559704505No Hit
GAGTAGCACTTAGTGCCAGTGCAATGTTGGCCCTCCACGCCATTCGCGCA25560.1299172616124989No Hit
GTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC24030.12214052412160988No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT24010.12203886742238257No Hit
GTGCAGCATGGGATGACCGTGTCAAAGGTTTTGTCTTCGGAACTGGGACC23700.1204631885843593No Hit
ATATTATGTAGACTCCGTGAAGGGCCGATTCACCCTCTCCAGAGACGATT23340.11863336799826778No Hit
GATTTTTACTGTGCAGCATGGGATGACCGTGTCAAAGGTTTTGTCTTCGG23060.11721017420908547No Hit
GTCTGAGGATGAGGCTGATTTTTACTGTGCAGCATGGGATGACCGTGTCA22990.11685437576178989No Hit
GTGCTACTCTGGTCCATTCTACTACTACGGTATGCATGTCTGGGGCCAAG21440.10897598157167357No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG19900.10114841573117091No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG19740.10033516213735243No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)