FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2160527
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGTCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG161370.7469011032956312No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC100280.46414601622659657No Hit
GACTATTACTGTGCAGCATACGATGACGCTTTGGGTGGCCCGCTGTTCGG98960.45803639574974064No Hit
GATGTATACTGGTACCAGCAGTTCCCAGGAACGGCCCCCACACTCCTCAT96560.44692799488273No Hit
GTAATACATGGCCGTGTCCTCGACTCTCAGGCTACTCATTTGTAGAAACA89290.4132787972564101No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACAGCGGGCCACCCAAA87550.40522520662782735No Hit
GTCCTGGGCCCAGTCTGTGCTGAATCAGCCACCCTCAGCGTCTGGGACCC81180.3757416593266365No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACAGCGG76510.3541265626395782No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGAATCAGCCACCCTCAGCGTCT73350.33950050149801414No Hit
ATCGTATGCTGCACAGTAATAGTCAGCCTCATCCTCGGACCGGAGCCCAC71370.3303360707827303No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC67410.3120072093521627No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT59280.274377501415164No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT57720.267157040851607No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGAATCAGCCAC57310.26525935570349274No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA55680.2577149001146479No Hit
ATGTATTACTGTGCGAGAGGAGATGGAGGTGAGGCGCAGTGGCCGGCCGC55430.25655777502433436No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA55370.2562800650026591No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCCTGGTACAGCCCGGGG54510.25229955469198023No Hit
GCATACGATGACGCTTTGGGTGGCCCGCTGTTCGGCGGAGGGACCAGGCT51970.24054316377439391No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA51130.23665522347094017No Hit
ACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACAGCGGGCCACCCAAAG50110.23193415310246066No Hit
GTATACATCATTAGTTCCGATGTTGGAGCTGCTTCCAGAACAAGACATGG48490.22443598251722843No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG45390.2100876313973396No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC44830.20749567119503712No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT44080.2040242959240963No Hit
GGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAG44060.20393172591687123No Hit
GTGCTGAATCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC43910.20323745086268302No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT43120.199580935577292No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA42940.1987478055122662No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG42530.19685012036415192No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA42220.19541528525216303No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT41910.19398045014017415No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC41630.1926844700390229No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA40720.18847253471028133No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39370.18222405922258783No Hit
GATTATTCCTATCGATGAGGAGTGTGGGGGCCGTTCCTGGGAACTGCTGG39170.18129835915033693No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG38500.17819726390829643No Hit
CAGTAATAGTCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGA38370.17759555886133338No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGA37950.17565158870960648No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT37780.17486474364819324No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT36750.17009738827610116No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC36710.169912248261651No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG36280.16792199310631156No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT36230.16769056808824884No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA36110.16713514804489832No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG36010.16667229800877287No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC35690.16519117789317142No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA35620.16486718286788363No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC35450.16408033780647036No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA34970.16185865763306823No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCC34790.16102552756804245No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT33910.15695244725013852No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG33390.1545456270622862No Hit
GCACAGTAATAGTCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAG33330.15426791704061094No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA33110.15324964696113497No Hit
CTATTACTGTGCAGCATACGATGACGCTTTGGGTGGCCCGCTGTTCGGCG32740.15153710182747082No Hit
GCCCCCACACTCCTCATCGATAGGAATAATCAGCGGCCCTCAGGGGTCCC32670.15121310680218297No Hit
GTGTAGGCTCTGGATTAAACCTCAGAACCTATGCCATGAGCTGGGTCCGC32190.14899142662878084No Hit
GTATACTGGTACCAGCAGTTCCCAGGAACGGCCCCCACACTCCTCATCGA31090.14390007623140094No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCC31080.1438537912277884No Hit
TTACTGTGCAGCATACGATGACGCTTTGGGTGGCCCGCTGTTCGGCGGAG31020.14357608120611315No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC30760.142372671112187No Hit
ATACTGGTACCAGCAGTTCCCAGGAACGGCCCCCACACTCCTCATCGATA30700.14209496109051173No Hit
CAGTAATACATGGCCGTGTCCTCGACTCTCAGGCTACTCATTTGTAGAAA30670.1419561060796741No Hit
GTATTACTGTGCGAGAGGAGATGGAGGTGAGGCGCAGTGGCCGGCCGCTC30520.14126183102548592No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA30360.1405212709676852No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG30190.13973442590627194No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC30150.13954928589182178No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG29210.1351984955522426No Hit
GTGCATACCACGCAGACTCCGTGAGGGGCCGGTTCACCATCTCCAGAGAC28210.1305699951909881No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT27910.12918144508261178No Hit
ATACATGGCCGTGTCCTCGACTCTCAGGCTACTCATTTGTAGAAACAGAG27860.1289500200645491No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT27370.1266820548875344No Hit
TCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGAATCAGCCA26970.12483065474303259No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG26750.12381238466355662No Hit
GGTACCAGCAGTTCCCAGGAACGGCCCCCACACTCCTCATCGATAGGAAT26560.12293296959491827No Hit
CTCCTCATCGATAGGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATT26470.12251640456240537No Hit
CTCCTGTGTAGGCTCTGGATTAAACCTCAGAACCTATGCCATGAGCTGGG26410.12223869454073011No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTG26320.1218221295082172No Hit
GTGTGGGGGCCGTTCCTGGGAACTGCTGGTACCAGTATACATCATTAGTT26230.1214055644757043No Hit
GGCCCAGTCTGTGCTGAATCAGCCACCCTCAGCGTCTGGGACCCCCGGGC25900.11987815935649032No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC25810.11946159432397743No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG25590.11844332424450145No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG25260.11691591912528748No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT24520.11349082885795919No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT24390.11288912381099611No Hit
GAACTAATGATGTATACTGGTACCAGCAGTTCCCAGGAACGGCCCCCACA24230.1121485637531954No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG23950.11085258365204416No Hit
CCCAAAGCGTCATCGTATGCTGCACAGTAATAGTCAGCCTCATCCTCGGA23820.11025087860508107No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGAATCAGCCACCCTCAGCGT23640.10941774854005526No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG23190.10733492337749077No Hit
GTCTTGTTCTGGAAGCAGCTCCAACATCGGAACTAATGATGTATACTGGT23160.10719606836665314No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA23100.10691835834497787No Hit
ATGCTGCACAGTAATAGTCAGCCTCATCCTCGGACCGGAGCCCACTGATG22810.10557609324021407No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC22460.10395611811377503No Hit
GGACTAAACTGAGAGAACTCACCATGGAGATAGGGCTGAGCTGGCTTGTT22380.10358583808487466No Hit
TATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGAT22220.10284527802707395No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT21980.10173443794037287No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC21800.10090130787534708No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)