FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646153
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA26020.4026910035239332No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20840.3225242318769703No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17840.27609559964899955No Hit
GTGTATTACTGTGCGAGAGTCTCCGGTGACTACGATAACAACTACTACTA14780.22873839477646934No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT12720.1968574006465961No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG12680.19623835221688982No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG12410.19205977531637244No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT12330.19082167845695988No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC10540.16311926122760398No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10290.15925020854193978No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG10040.15538115585627552No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC9010.13944065879133888No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT8400.13000017023831817No Hit
AATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGA8380.12969064602346503No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC8270.12798826284177278No Hit
CCCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATACCGT8220.12721445230463993No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACTCAGGCTG7930.12272635118926942No Hit
GTATTTATTACTTCCATCATATGATATAACTGCCACCCACTCCAGCCCCT7810.12086920590015059No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT7240.11204776577683614No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT7180.11111919313227672No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC7110.11003585838029073No Hit
ATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACAGCG7020.10864299941345161No Hit
CAGTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATA6910.10694061623175935No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG6750.10446442251293424No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG6510.10075013193469658No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12300.056.229551
ATCAACG12950.051.714213
TCAACGC12950.051.7102054
CAACGCA13200.050.7308435
ACACCGT450.00990195548.003386
TATCAAC14150.047.8374562
AACGCAG14150.047.3248866
CGCAGAG16550.040.452678
GGTATCA5650.038.2535061
GCAGAGT19200.035.2442979
TAGGTGG9100.034.815645
ATAGGTG9450.032.0022544
GAATACG2301.0804797E-931.2992849
GTACATG14600.030.1006181
GTGTAGA2901.2732926E-1129.8113541
CAGAGCG7050.029.612239
TACATGG14700.028.9022312
GTGCTAG2752.2919266E-1028.8176421
ACGCCGC1000.00627954228.8020296
ACATGGG15150.028.0437493
Produced by FastQC (version 0.11.5)