FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646153
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA25660.39711956765657674No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG23390.36198856927074546No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16030.24808365820479053No Hit
GTGTATTACTGTGCGAGAGTCTCCGGTGACTACGATAACAACTACTACTA14290.22115505151256742No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG13920.2154288535377844No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG12420.19221453742379901No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT12210.18896453316784106No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT10810.16729783812812135No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC10780.16683355180584164No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG8970.1388216103616326No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT8930.13820256193192634No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC8910.1378930377170732No Hit
AATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGA8770.13572636821310124No Hit
GTATTTATTACTTCCATCATATGATATAACTGCCACCCACTCCAGCCCCT8420.1303096944531713No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC8400.13000017023831817No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC8350.12922635970118532No Hit
CCCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATACCGT7870.12179777854471No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT7260.11235728999168927No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACTCAGGCTG7250.1122025278842627No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC7190.11127395523970328No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT6930.10725014044661249No Hit
ATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACAGCG6910.10694061623175935No Hit
CCGTAGTAGTAGTAGTTGTTATCGTAGTCACCGGAGACTCTCGCACAGTA6710.10384537408322796No Hit
CAGTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATA6590.10198822879410913No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA6490.10044060771984345No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC10300.045.4486434
ATCAACG10500.044.5829543
TATCAAC10850.043.8085632
CAACGCA10900.043.6076055
GTATCAA11450.043.4133151
ATAGGTG9150.042.5027924
GAATACG2050.042.144199
AACGCAG11550.041.76416
CGCAGAG11900.039.328778
TAGGTGG10550.037.5452545
GGTATCA6250.035.7323041
GTGCTAG3050.035.429971
GTGGTCA11000.035.3463868
TCTACAC5300.035.329893
CCGTTGA3600.033.998269
CCCCGTT3650.033.532537
ACCCCGT4100.033.364156
CCCGTTG3800.032.2113768
ACAGCCG12550.031.5546178
AGGTGGT12600.031.4269666
Produced by FastQC (version 0.11.5)