FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2396196
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTCTGAGCTGACACAGCCACCCTCGGTGTCAGTGTC165980.6926812330877775No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC110320.46039639495266665No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT90260.3766803717225135No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA78590.32797817874664675No Hit
GACTACTACTGTTCCTCAGCAGACATCACTGGTAATGAGAGGATTTTCGG74440.31065906127879356No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT73310.3059432533899564No Hit
GTAGTAGTCAGCTTCATCCTCCACCTGGGCCCCACTGATAGTCAAGGTGA71520.298473079831533No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA71240.29730456106261755No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCCTCTCATTACCA69140.2885406702957521No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT68860.2873721515268367No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA68840.2872886859004856No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC67180.2803610389133443No Hit
TCTATGAGGACAACAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGCC63260.2640017761485287No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC63150.26354271520359773No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG58720.2450550789668291No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG57490.23992194294623648No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG56470.23566519600233035No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCCT56400.23537306631010152No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTG55960.2335368225303773No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA54670.22815328963073137No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG54370.22690130523546487No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT54150.22598318334560277No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA53670.22398000831317638No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG53160.22185163484122333No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA52840.22051618481960575No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA52720.22001539106149912No Hit
CTCTGAGGCCTCCTCTGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCC48730.2033639986044547No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTCTGAGCTGACACAGCCACCCTCG47890.19985844229770855No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT46570.19434971095853595No Hit
CCCCTGCGCTGGTCATCTATGAGGACAACAAACGACCCTCCGGGATCCCT45910.19159534528894964No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45610.19034336089368314No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT45130.18834018586125675No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC44580.18604488113660153No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA44380.18521022487309052No Hit
CCTCTGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACG43180.18020228729202453No Hit
GGCCAGGCCCCTGCGCTGGTCATCTATGAGGACAACAAACGACCCTCCGG41720.17410929656839425No Hit
GGTCATCTATGAGGACAACAAACGACCCTCCGGGATCCCTGAGAGATTCT41470.1730659762390055No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG41370.17264864810725No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC41010.17114626683293022No Hit
GACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTACTGTTCCT40800.17026987775624364No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG40750.1700612136903659No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC39830.1662217948782153No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC38690.1614642541762026No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG37860.15800043068263198No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGAATCACCTGCTCTGGAGATGC37770.15762483536405203No Hit
CCCCAGGACAAACGGCCAGAATCACCTGCTCTGGAGATGCATTCCCAAAA37640.15708230879276988No Hit
CCTTGACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTACTGT37010.15445314156271023No Hit
GCTCTGGAGATGCATTCCCAAAAAAATATGCTTATTGGTACCAGCAGAAG36960.15424447749683248No Hit
ATAATATACGGCCGTGTCGTCAGATCTCAGGCTGTTCAGTTCCATGTAGA36430.15203263839852832No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT35930.1499459977397508No Hit
GTACATGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCC35830.14952866960799535No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC35440.1479010898941489No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA34830.14535538829044034No Hit
CTACTGTTCCTCAGCAGACATCACTGGTAATGAGAGGATTTTCGGCGGAG32700.13646629908404823No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT32670.13634110064452157No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCCTCTCATTACCAG32660.13629936783134602No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG32630.13617416939181937No Hit
GTATCAACGCAGAGTACATGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAA32280.13471352093067512No Hit
GTCTACATGGAACTGAACAGCCTGAGATCTGACGACACGGCCGTATATTA31660.132126086513791No Hit
CTACTACTGTTCCTCAGCAGACATCACTGGTAATGAGAGGATTTTCGGCG30980.12928825521785364No Hit
TAGTCAAGGTGACCATTGCCCCTGACGTGGAGGCAGAGAATCTCTCAGGG30810.12857879739386927No Hit
CATTACCAGTGATGTCTGCTGAGGAACAGTAGTAGTCAGCTTCATCCTCC30630.1278276067567094No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC30020.12528190515300083No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG29890.1247393785817187No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT29650.12373779106550549No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT29520.12319526449422334No Hit
GGTAATGAGAGGATTTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCA28880.12052436445098816No Hit
CTGATAGTCAAGGTGACCATTGCCCCTGACGTGGAGGCAGAGAATCTCTC28500.11893851755031726No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTCTGA28360.11835425816585955No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT28270.1179786628472796No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC28210.1177282659682263No Hit
GAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAG28160.11751960190234856No Hit
CTGGTAATGAGAGGATTTTCGGCGGAGGGACCAAGCTGACCGTCCTACGT28050.11706054095741751No Hit
GTATATTATTGTGCGAGAGATGTCATTGGGAGAAGTAGTTGGATGGTGGG27710.1156416253094488No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA27340.11409751122195347No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACA26880.11217780181587816No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGC25710.10729506267433883No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGT25370.10587614702637013No Hit
ACAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGCCTCCACGTCAGGG25300.10558401733414127No Hit
TCCTCCACCTGGGCCCCACTGATAGTCAAGGTGACCATTGCCCCTGACGT24900.10391470480711928No Hit
GCGCAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATTTTTTT24900.10391470480711928No Hit
CGTCAGGGGCAATGGTCACCTTGACTATCAGTGGGGCCCAGGTGGAGGAT24560.10249578915915059No Hit
GGTGGAGGATGAAGCTGACTACTACTGTTCCTCAGCAGACATCACTGGTA24530.10237059071962393No Hit
TAGATGACCAGCGCAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGC24270.10128553757705964No Hit
CCCTGAGAGATTCTCTGCCTCCACGTCAGGGGCAATGGTCACCTTGACTA24230.10111860632435743No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC24110.10061781256625084No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC24040.100325682874022No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA23990.10011701880814423No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17800.097.12381
GTATCAA48300.079.639511
ATCAACG60950.062.2585643
TCAACGC61500.061.7017824
TATCAAC64850.059.1793752
CAACGCA67400.056.299415
AACGCAG69250.054.7942436
CGCAGAG74850.050.7898758
ACGCAGA83050.045.77517
GCAGAGT97500.039.1386879
TATTGAG56200.036.0017135
TTATTGA60650.033.5983434
GTACATG63850.032.1527671
CAGAGTA80750.031.6534359
TATAACG1153.2935062E-431.3058342
ACATGGG65100.031.0804673
ATTGAGG26250.030.720826
ATTGAGT40800.029.8243266
TTGAGGG26450.029.3990357
CATGGGA18000.029.2019964
Produced by FastQC (version 0.11.5)