FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2396196
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTCTGAGCTGACACAGCCACCCTCGGTGTCAGTGTC140320.5855948344793164No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC95660.39921609083731047No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT80430.33565701637094797No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA69270.28908319686703426No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA66840.27894212326537565No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT65040.2714302168937766No Hit
GACTACTACTGTTCCTCAGCAGACATCACTGGTAATGAGAGGATTTTCGG63950.2668813402576417No Hit
GTAGTAGTCAGCTTCATCCTCCACCTGGGCCCCACTGATAGTCAAGGTGA63210.263793112082651No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCCTCTCATTACCA60190.25118980250363493No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT60180.2511480696904594No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC58650.2447629492746002No Hit
TCTATGAGGACAACAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGCC58080.2423841789235939No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG54970.22940527402599786No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA54720.2283619536966091No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC53340.22260282547838323No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG50490.2107089737233515No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT50050.2088727299436273No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA49940.2084136689986963No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG49240.2054923720764078No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA49180.2052419751973545No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG49090.2048663798787745No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG47900.19990017511088407No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA47760.19931591572642637No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTG47620.1987316563419687No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCCT47110.19660328287001566No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA46750.19510090159569585No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41990.17523608252413408No Hit
CCCCTGCGCTGGTCATCTATGAGGACAACAAACGACCCTCCGGGATCCCT41500.17319117467853212No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT41420.17285731217312775No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTCTGAGCTGACACAGCCACCCTCG41380.17269038092042555No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA40380.16851709960287053No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT40020.16701471832855075No Hit
CCTCTGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACG38540.16083826197856937No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC38390.1602122697809361No Hit
CTCTGAGGCCTCCTCTGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCC38280.15975320883600508No Hit
GACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTACTGTTCCT37330.15578859158432784No Hit
GGCCAGGCCCCTGCGCTGGTCATCTATGAGGACAACAAACGACCCTCCGG37180.15516259938669458No Hit
GGTCATCTATGAGGACAACAAACGACCCTCCGGGATCCCTGAGAGATTCT36980.1543279431231836No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC36070.15053025712420853No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC35670.14886094459718655No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG35350.14752549457556896No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG35350.14752549457556896No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC33970.14176636635734305No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG33800.14105690853335873No Hit
CCTTGACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTACTGT33700.1406395804016032No Hit
GCTCTGGAGATGCATTCCCAAAAAAATATGCTTATTGGTACCAGCAGAAG33430.13951279444586337No Hit
GTATCAACGCAGAGTACATGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAA32870.13717575690803258No Hit
GTACATGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCC32680.13638283345769714No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT32620.13613243657864382No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGAATCACCTGCTCTGGAGATGC32550.13584030688641496No Hit
ATAATATACGGCCGTGTCGTCAGATCTCAGGCTGTTCAGTTCCATGTAGA31570.13175049119521107No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA31460.13129143025028003No Hit
CCCCAGGACAAACGGCCAGAATCACCTGCTCTGGAGATGCATTCCCAAAA31280.1305402396131201No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC31160.13003944585501354No Hit
TAGTCAAGGTGACCATTGCCCCTGACGTGGAGGCAGAGAATCTCTCAGGG28950.12081649414321699No Hit
CTGATAGTCAAGGTGACCATTGCCCCTGACGTGGAGGCAGAGAATCTCTC28890.1205660972641637No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCCTCTCATTACCAG28810.12023223475875931No Hit
CTACTGTTCCTCAGCAGACATCACTGGTAATGAGAGGATTTTCGGCGGAG28230.11781173159457742No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG28000.11685187689153977No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT27660.11543296124357105No Hit
GTCTACATGGAACTGAACAGCCTGAGATCTGACGACACGGCCGTATATTA27520.11484870185911335No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT27500.11476523623276226No Hit
CATTACCAGTGATGTCTGCTGAGGAACAGTAGTAGTCAGCTTCATCCTCC27450.11455657216688452No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT27400.11434790810100676No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG27360.11418097684830456No Hit
GGTAATGAGAGGATTTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCA27250.11372191590337351No Hit
CTACTACTGTTCCTCAGCAGACATCACTGGTAATGAGAGGATTTTCGGCG26590.11096755023378721No Hit
CTGGTAATGAGAGGATTTTCGGCGGAGGGACCAAGCTGACCGTCCTACGT26260.10959036739899408No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC25900.10808798612467428No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT25710.10729506267433883No Hit
GTATATTATTGTGCGAGAGATGTCATTGGGAGAAGTAGTTGGATGGTGGG25570.10671080328988113No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTCTGA25510.10646040641082784No Hit
GAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAG25050.10454069700475255No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA24930.10403990324664594No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC24890.10387297199394374No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48500.084.322951
GGTATCA17200.083.722231
ATCAACG60500.066.4159553
TCAACGC61300.065.5491944
TATCAAC62000.065.157562
CAACGCA70950.056.6337665
AACGCAG72550.055.882226
CGCAGAG75000.053.5766568
ACGCAGA82400.048.7651637
GCAGAGT99600.040.4077389
TATTGAG54300.036.2039875
GTGCTAG4850.034.144861
TTATTGA59500.033.0399444
ATTGAGG25300.032.7325636
AATCCGC6600.031.6414766
TTGAGGG27500.030.1139587
GTACATG67500.030.0804651
GAATACG4400.029.4531579
ACATGGG69450.028.5137313
CAGAGTA79950.028.4564469
Produced by FastQC (version 0.11.5)