FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458762
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19010.4143760817155736No Hit
GTAATATATGGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATAGA17990.392142330881808No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15580.3396096450883029No Hit
ATATATTACTGCGCAAGAAGAGTCCCGTACAGTAGTTCCTCCGCCTATTT12660.27596008387791493No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT12570.2739982823337591No Hit
ATATATGGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATAGACTG12030.2622274730688243No Hit
ATACTACGCAGACCCCGTGAGGGGCCGGTTCACCATCTCCAGAGACAGGT11200.24413530327272093No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG11070.24130158993116257No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT11020.24021170018440935No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG9600.20925883137661797No Hit
GCCATATATTACTGCGCAAGAAGAGTCCCGTACAGTAGTTCCTCCGCCTA8910.19421835287142353No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC8900.1940003749220729No Hit
GCGTAGTATGTATCGCCACCACTATCACTAATAGCCGAGACCCACTCCAG8350.18201158770778747No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT7770.16936886664545014No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7490.16326548406363212No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAATAGTCAAAATAGG6890.15018680710259352No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG6630.14451938041947676No Hit
CTCCAGAGACAGGTCCACAGTCTATCTGCAAATGAACAGCCTGAGAGCCG6600.14386544657142483No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGTCATGCCATGAGCTGGG6380.13906993168571066No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT6380.13906993168571066No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC6150.13405643885064586No Hit
GTATGTATCGCCACCACTATCACTAATAGCCGAGACCCACTCCAGCCCCT6040.13165868140778877No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT5990.13056879166103558No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCT5920.12904294601558106No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTGCAGCCTGGGG5840.1272991224207759No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC5530.12054180599090597No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC5460.11901596034545145No Hit
GTCTATCTGCAAATGAACAGCCTGAGAGCCGAAGACACGGCCATATATTA5410.11792607059869824No Hit
CCTCATATATGGTGCATCCACCAGGGCCACTGGCATCCCAGACAGGTTCA5340.11640022495324374No Hit
GTCTCTGGAGATGGTGAACCGGCCCCTCACGGGGTCTGCGTAGTATGTAT5310.11574629110519181No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC5290.11531033520649052No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC5210.11356651161168536No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT5170.11269459981428279No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT5010.10920695262467248No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA4970.10833504082726991No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT4830.1052833495363609No Hit
CCCTCAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGTCATGCC4720.10288559209350383No Hit
GTGGATGCAGAGGGGGCAGCCCTTGTCCCCGGAGAAGTATGTGACCAGCG4700.10244963619480253No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGC4680.10201368029610124No Hit
TTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGC4670.1017957023467506No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9750.072.40531
GGTATCA3950.063.8292581
ATCAACG10850.063.7090263
TCAACGC10850.063.7090264
CAACGCA11200.061.7181175
TATCAAC11800.059.1901172
AACGCAG11800.058.5799066
CGCAGAG12500.055.29348
GCAGAGT14250.049.008239
CCGTGCA855.5608958E-542.3509529
CAGAGCG7200.034.998369
AGGCCGT850.00283415133.8844576
GTCATAG8250.031.433841
TAGGTGG7900.030.9892655
ATAGGTG8050.030.4118254
GACTGAT9950.030.3905337
ACGCAGA23450.030.088147
AGGTGGT8650.029.9671786
TCTACAC2253.0036972E-828.8017883
TTACACC2003.4487675E-728.8017884
Produced by FastQC (version 0.11.5)