FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458762
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20670.4505604213077805No Hit
GTAATATATGGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATAGA18240.3975917796155741No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15360.3348141302025887No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT12230.2665870320558372No Hit
ATATATTACTGCGCAAGAAGAGTCCCGTACAGTAGTTCCTCCGCCTATTT12210.26615107615713596No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG12210.26615107615713596No Hit
ATATATGGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATAGACTG11460.24980272995583766No Hit
ATACTACGCAGACCCCGTGAGGGGCCGGTTCACCATCTCCAGAGACAGGT11110.24217350172856517No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT9800.21361839036363078No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC9350.20380938264285184No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG8420.183537433353242No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC7920.17263853588570982No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAATAGTCAAAATAGG7900.17220257998700852No Hit
GCCATATATTACTGCGCAAGAAGAGTCCCGTACAGTAGTTCCTCCGCCTA7760.1691508886960995No Hit
GCGTAGTATGTATCGCCACCACTATCACTAATAGCCGAGACCCACTCCAG7200.15694412353246345No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT7180.15650816763376216No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT6640.1447373583688274No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTGCAGCCTGGGG6470.14103173322986645No Hit
CTCCAGAGACAGGTCCACAGTCTATCTGCAAATGAACAGCCTGAGAGCCG6410.1397238655337626No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG6270.1366721742428536No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC6210.13536430654674975No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGTCATGCCATGAGCTGGG6170.13449239474934715No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC5890.12838901216752913No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCT5860.1277350783194772No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA5830.12708114447142527No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT5470.11923393829480208No Hit
GGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTG5370.11705415880129566No Hit
CCCTCAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGTCATGCC5370.11705415880129566No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC5320.11596426905454245No Hit
GTCTATCTGCAAATGAACAGCCTGAGAGCCGAAGACACGGCCATATATTA5320.11596426905454245No Hit
CCTCATATATGGTGCATCCACCAGGGCCACTGGCATCCCAGACAGGTTCA5230.11400246751038666No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT5090.11095077621947763No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT5070.11051482032077636No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA5000.10898897467532184No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCG4980.10855301877662055No Hit
GTATGTATCGCCACCACTATCACTAATAGCCGAGACCCACTCCAGCCCCT4890.10659121723246476No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT4830.1052833495363609No Hit
GTCTCTGGAGATGGTGAACCGGCCCCTCACGGGGTCTGCGTAGTATGTAT4820.10506537158701025No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC4740.10332154799220512No Hit
GCCTGGGGGGTCCCTCAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA4720.10288559209350383No Hit
CATATATGGTGCATCCACCAGGGCCACTGGCATCCCAGACAGGTTCAGTG4600.1002698567012961No Hit
CCTTTAGCAGTCATGCCATGAGCTGGGTCCGTCAGGCTCCAGGGAAGGGG4590.10005187875194545No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)