FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630433
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAATTCCATGTAGG54050.3315070291143518No Hit
GTGTATTACTGTGCGAGAGACGGGGTAGTGGTGGAAGCTGCTGCTACTCC42060.2579682820453217No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC41170.25250960941050626No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT34760.21319489975975708No Hit
ATAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG34250.21006689633980666No Hit
GTCTGGGGCTGAACTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA34030.20871756153120058No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA33270.20405622310147056No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC32010.19632821465218137No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC31850.1953468802459224No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT31550.19350687823418686No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA31500.19320021123223094No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT31450.19289354423027502No Hit
GACCTAGGACGGTCATCTTGGTCCCTCCGCCGAACACAAGACCACCCAGG30980.19001087441188935No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT30940.18976554081032462No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC30090.18455220177707393No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG26800.16437351304837428No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA25080.15382416818109054No Hit
GCCTTGGGCTGACCTAGGACGGTCATCTTGGTCCCTCCGCCGAACACAAG24400.14965349695448998No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG24080.1476908281419721No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC22630.13879748508525036No Hit
GATTATTATTGTGCAGCATGGGATGACAGCCTGGGTGGTCTTGTGTTCGG22550.13830681788212087No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT22450.13769348387820904No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT22250.13646681587038537No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21640.1327254784465231No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA21530.13205081104222005No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA20770.12738947261249003No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG20540.12597880440349282No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT19990.12260546738197767No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC19730.12101079897180687No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19650.1205201317686774No Hit
CCTCTGAGGAGCTGCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19530.11978413096398319No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT18990.11647212734285924No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA18730.11487745893268844No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG18530.11365079092486474No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18500.11346679072369119No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGACAGAGGGTCAC18410.11291479012017053No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATTATGCCTATGTTTGAT18100.1110134547080438No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC18030.11058412090530552No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG17890.10972545329982894No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA17720.1086827854931788No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17620.10806945148926696No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA17450.10702678368261682No Hit
GCATGGGATGACAGCCTGGGTGGTCTTGTGTTCGGCGGAGGGACCAAGAT17250.10580011567479312No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT17150.10518678167088129No Hit
GTATTACTGTGCGAGAGACGGGGTAGTGGTGGAAGCTGCTGCTACTCCTA16610.10187477804975735No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA16540.10144544424701904No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG16460.10095477704388955No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9800.065.7682651
TCAACGC27450.060.1823774
ATCAACG27450.059.9384043
CAACGCA28200.058.5817835
TATCAAC28850.057.064822
AACGCAG29500.056.223666
CGCAGAG34650.047.867188
CAGCGCT10050.042.660429
ACGCAGA41350.040.4569787
GTATCAA50400.035.807171
GGCAGCG12350.034.732617
GCAGCGC12450.034.453638
GTACATG37350.032.5955471
ACATGGG37750.030.6985443
GTGTACG1453.797252E-529.6335181
TATTGAG18350.026.8913565
CAGAGTA40500.026.2888269
GCAGAGT68050.025.3063139
GTATGTA20100.025.2966061
GTACACG18250.024.6875175
Produced by FastQC (version 0.11.5)