FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630433
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAATTCCATGTAGG52920.324576354870148No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC39240.2406722631350077No Hit
GTGTATTACTGTGCGAGAGACGGGGTAGTGGTGGAAGCTGCTGCTACTCC38070.23349625528923912No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC37170.2279762492540325No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT36470.2236829112266496No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT33920.20804289412689755No Hit
GTCTGGGGCTGAACTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA32580.19982421847447887No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA30880.18939754040797752No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC30210.18528820258176815No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT29780.18265086636494723No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA28550.17510685811683155No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT27460.16842151747419246No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC25700.157626839005344No Hit
GACCTAGGACGGTCATCTTGGTCCCTCCGCCGAACACAAGACCACCCAGG25540.15664550459908502No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC23820.1460961597318013No Hit
GATTATTATTGTGCAGCATGGGATGACAGCCTGGGTGGTCTTGTGTTCGG23220.14241615570833024No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG23040.14131215450128892No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22360.1371414832746884No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21640.1327254784465231No Hit
GCCTTGGGCTGACCTAGGACGGTCATCTTGGTCCCTCCGCCGAACACAAG20910.12824814021796663No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA20560.12610147120427517No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT20420.1252428035987986No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA20170.12370946858901899No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG20170.12370946858901899No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGACAGAGGGTCAC20160.12364813518862781No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT20120.12340280158706306No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT18920.11604279354012093No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC18300.1122401227158675No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT18230.11181078891312922No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18180.11150412191117329No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG18100.1110134547080438No Hit
CCTCTGAGGAGCTGCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18040.1106454543056967No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT17970.11021612050295841No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17670.10837611849122289No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA17290.10604544927635788No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG17000.10426678066501353No Hit
ATAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG16550.10150677764741024No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)