FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633764
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA23440.3698537625993272No Hit
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA18490.29174897911525427No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT17960.28338624472201007No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG17410.27470793544600197No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG13570.21411755795532722No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11670.18413794409275377No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT11610.18319121944446196No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11110.1753018473753637No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG10580.16693911298211953No Hit
GTGTATTACTGTGCGAGAGAGGCCTGTGGTGGTGATTGCTATGCCTACTA10570.16678132554073755No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT10530.1661501757752097No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG9610.15163373116806886No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG9570.15100258140254103No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC8780.13853737353336573No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG8070.1273344651952462No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT7620.12023403033305774No Hit
AATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGA7370.1162893442985086No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT7070.11155572105704963No Hit
GTAGTAGGCATAGCAATCACCACCACAGGCCTCTCTCGCACAGTAATACA6870.10839997222941032No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC6750.10650652293282674No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGTG6800.039.1740464
TAGGTGG7100.037.5188065
GATCGTC1001.4491842E-435.997777
GTGGTCA7900.033.7194338
AGGTGGT9200.029.7372916
GTGCTAG3201.8189894E-1229.2643531
CAGAGCG7000.028.7982169
CATAGGT9350.028.4902153
GTACTAG1050.00795196727.4420281
GTCATAG13450.027.3145081
GAATACG2259.66993E-725.5984139
CATGGGG8450.024.708534
GTATAGG2051.3551584E-524.5974271
TAGGACA2401.6965569E-623.9985144
TGGTCAG12750.023.7161779
GACAGCG8550.023.5774887
CTAGAGA3702.8376235E-1023.3499054
ACCAGCG7300.022.6835278
CCCCGTT2552.8735394E-622.5868387
GCACACA9250.022.5715732
Produced by FastQC (version 0.11.5)