FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633764
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA22270.35139263195763726No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT19370.30563427395686726No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19230.3034252497775197No Hit
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA17400.27455014800462No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14180.2237425918796271No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT12700.2003900505550962No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG11550.1822444947961702No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT10860.17135716134081455No Hit
GTGTATTACTGTGCGAGAGAGGCCTGTGGTGGTGATTGCTATGCCTACTA10250.16173212741651466No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT9480.14958249443010332No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG9450.14910913210595741No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC9420.14863576978181153No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG9370.1478468325749017No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT7870.12417871636760686No Hit
GTAGTAGGCATAGCAATCACCACCACAGGCCTCTCTCGCACAGTAATACA7270.11471146988468893No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC7040.11108235873290373No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC6750.10650652293282674No Hit
AATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGA6680.10540201084315297No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG6400.10098396248445793No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGTG8900.038.0237854
GAATACG1951.8371793E-1036.918529
AATCCGC1051.9312499E-434.2814836
GTGGTCA9900.033.453068
TAGGTGG10250.033.0157745
GTATTAG3400.031.7733271
TAGTACC1851.744229E-731.1362114
CAGAGCG7150.029.1991969
TGCTAGA3700.029.1901972
GCACACA7900.028.254142
GACAGCG7900.027.3384027
CATGGGG8150.026.503984
GTCATAG15400.026.1889251
AGGTGGT12750.025.9732656
TGGTCAG14700.025.9559829
CATAGGT13050.025.9319293
CAGCCGT10300.025.8608889
ACAGCCG10750.025.4500338
TATAGGG1701.08008E-425.4126422
CTAGAGA4001.8189894E-1225.2008694
Produced by FastQC (version 0.11.5)