FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857077
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT30800.35936094423254855No Hit
GCCTATATGGAGCTGAGCAGCCTGACATCTGACGACACGGCCTTTTATTA30190.35224373072664417No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT22650.26427030476841634No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21530.25120263406905097No Hit
CCATATAGGCTGTGCTCGTGGATTCGTCCGCGGTAAGGGTGACTCTGTCC21180.24711898697549928No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC19990.23323458685742354No Hit
GTCTGGGGCTGAGGTGAGGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA18980.2214503481017458No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18580.21678332285197244No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18100.21118289255224443No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT17810.20779929924615875No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC17460.20371565215260706No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17380.2027822471026524No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17350.20243222020891938No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17250.20126546389647604No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16020.18691436125342298No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGC15950.18609763183471262No Hit
ATATGGAGCTGAGCAGCCTGACATCTGACGACACGGCCTTTTATTATTGT15900.18551425367849098No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT15420.17991382337876294No Hit
CATATAGGCTGTGCTCGTGGATTCGTCCGCGGTAAGGGTGACTCTGTCCT13760.1605456685922035No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG13590.15856218286104984No Hit
TTCCAGGACAGAGTCACCCTTACCGCGGACGAATCCACGAGCACAGCCTA13550.15809548033607249No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13410.1564620214986518No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG13120.15307842819256612No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12830.14969483488648044No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG12470.1454945121616844No Hit
CCTATACTATCAGCTGGGTGCGACAGGCCCCTGGACAAGAGCTTGAGTGG12340.1439777289555081No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT11870.13849397428702437No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAACTCCTTAGCCTGGTACCAAC11640.1358104347684047No Hit
GCTCATCACCATGGGCTGGACCTCGAGGTTCCTCTTTCTGGTGGCAGCAG11450.13359359777476237No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11420.13324357088102937No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11100.12950995068121066No Hit
GTCCTGGAACTTCTGTGCGTACGTGGTTGCATGAAAGACAGGATTGATCC10920.12740978931881267No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC10870.126826411162591No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA10710.12495960106268164No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10660.12437622290645999No Hit
CACCTATACTATCAGCTGGGTGCGACAGGCCCCTGGACAAGAGCTTGAGT10650.12425954727521564No Hit
TTATTATTGTGCGAAGAGTAGCCGAGATGTTTTGACTGGTTATCTCCATG10620.12390952038148265No Hit
GCCTTTTATTATTGTGCGAAGAGTAGCCGAGATGTTTTGACTGGTTATCT10610.12379284475023832No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCGTAGCGTCTGG10530.12285943970028364No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT10510.12262608843779496No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGGCTGGACCTC10510.12262608843779496No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT10490.12239273717530631No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10300.12017590018166396No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10160.11854244134424329No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10140.11830909008175461No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG10130.11819241445051028No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA9740.11364206483198125No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA9640.11247530851953792No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9410.10979176900091824No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC9320.10874168831971924No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGAG8950.10442468996367886No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC8940.10430801433243456No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8810.10279123112625821No Hit
TTATTACTGTCAGCGTAGCGTCTGGCCCCGTGGCTCTTTCGGCCCTGGGA8790.10255787986376953No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG8680.10127444792008186No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8660.1010410966575932No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG8590.10022436723888285No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13900.078.3075941
GGTATCA5150.076.4769441
TCAACGC17300.062.3906564
ATCAACG17700.061.0127373
CAACGCA17700.060.9806985
TATCAAC18100.060.4687772
AACGCAG19000.056.8017046
CGCAGAG21950.049.167768
GCAGAGT24950.042.9618239
CAGCGCT5150.041.6270879
ACGCAGA27100.039.8240747
AACACCG750.001590133938.123035
GCAGCGC6250.036.5938388
GGCAGCG6600.034.6532557
GTATAGA1906.1190804E-933.9206351
GTACATG22650.032.5644761
GAATACG1801.4667239E-731.7599249
ACATGGG22550.031.7154083
TACATGG23300.030.7016962
CATTAGC5400.030.4419258
Produced by FastQC (version 0.11.5)