FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857077
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT29890.34874346178931415No Hit
GCCTATATGGAGCTGAGCAGCCTGACATCTGACGACACGGCCTTTTATTA27880.325291659909203No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT20310.23696820705724222No Hit
CCATATAGGCTGTGCTCGTGGATTCGTCCGCGGTAAGGGTGACTCTGTCC19980.2331179112261792No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19330.22553399519529752No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19060.2223837531517005No Hit
GTCTGGGGCTGAGGTGAGGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA17810.20779929924615875No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC17050.19893195127158939No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA16880.19694846554043569No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16430.19169806213444066No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT15850.1849308755222693No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15720.18341409231609296No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15450.18026385027249595No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15430.18003049901000726No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGC14690.17139650229792658No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT14020.16357923500455618No Hit
ATATGGAGCTGAGCAGCCTGACATCTGACGACACGGCCTTTTATTATTGT13970.1629958568483345No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13340.1556452920799415No Hit
CATATAGGCTGTGCTCGTGGATTCGTCCGCGGTAAGGGTGACTCTGTCCT12410.14479445837421842No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG12060.14071081128066673No Hit
TTCCAGGACAGAGTCACCCTTACCGCGGACGAATCCACGAGCACAGCCTA11770.13732721797458106No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11430.13336024651227368No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG11380.132776868356052No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11170.13032668009992102No Hit
GTCCTGGAACTTCTGTGCGTACGTGGTTGCATGAAAGACAGGATTGATCC11090.12939327504996634No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCGTAGCGTCTGG10750.125426303587659No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA10690.12472624980019299No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10550.12309279096277231No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10500.12250941280655064No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10460.12204271028157329No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10450.12192603465032896No Hit
CCTATACTATCAGCTGGGTGCGACAGGCCCCTGGACAAGAGCTTGAGTGG10390.12122598086286296No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT10330.12052592707539696No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10290.12005922455041963No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAACTCCTTAGCCTGGTACCAAC10280.11994254891917529No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT10270.11982587328793096No Hit
TTATTATTGTGCGAAGAGTAGCCGAGATGTTTTGACTGGTTATCTCCATG10020.1169089825068226No Hit
GCTCATCACCATGGGCTGGACCTCGAGGTTCCTCTTTCTGGTGGCAGCAG10010.11679230687557828No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGGCTGGACCTC9670.11282533541327092No Hit
CACCTATACTATCAGCTGGGTGCGACAGGCCCCTGGACAAGAGCTTGAGT9600.11200860599456057No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9500.11084184968211724No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG9320.10874168831971924No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT9110.10629150006358822No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG9080.10594147316985521No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9050.10559144627612221No Hit
TTATTACTGTCAGCGTAGCGTCTGGCCCCGTGGCTCTTTCGGCCCTGGGA8890.10372463617621286No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8850.10325793365123553No Hit
GCCTTTTATTATTGTGCGAAGAGTAGCCGAGATGTTTTGACTGGTTATCT8760.10220785297003653No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA8690.1013911235513262No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10400.071.3204351
ATCAACG11750.061.2875483
TCAACGC11750.061.2875484
TATCAAC12300.059.7179872
CAACGCA13150.054.7626385
AACGCAG13650.052.741296
GGTATCA3950.049.2239841
CGCAGAG15250.046.2636228
GCAGAGT18450.038.629789
ACGCAGA19700.036.178657
CATTAGC4750.033.34368
GTACATG20950.032.3112641
TACATGG20950.032.3112642
ACATGGG21000.032.234333
ATTAGCA5250.028.7967439
ACATTAG5300.028.5250767
GTATTAT2354.5865818E-827.5793951
CAGCGCT5750.027.5447129
TTAGGAC11850.027.346662
TAGGACC11900.027.2317583
Produced by FastQC (version 0.11.5)