FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1056949
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAATACTACTCAGAATCCCTGCAGGACCGATTCACCATCTCCAGAGA60520.5725914873849164No Hit
GTAATAAACAGCCGTGTCCTCAGGTCTCAGGCTGTCCATTTGCAGATACA53300.5042816635428956No Hit
GTTTATTACTGTGCGAGAGAGGGTCAATATAGCAGCAGGTGGTTTGCGAC44430.4203608688782524No Hit
ATATAGCAGCAGGTGGTTTGCGACTTCCAATTTTGACCACTGGGGCCAGG39050.3694596428020652No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT33580.3177069092264622No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGTTGGTGGAGTCTGGGGGAGGCG32290.30550196840150284No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT29230.27655071342136656No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC28430.2689817578710042No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT27790.26292659343071423No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA27700.26207508593129847No Hit
GTAGTTATACTATCCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAG27340.25866905593363543No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA27130.2566822051016653No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGGCCAG26750.25308695121524316No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC26440.2501539809394777No Hit
TAGTTATACTATCCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGT24340.23028547261977633No Hit
ATATTGACCCTCTCTCGCACAGTAATAAACAGCCGTGTCCTCAGGTCTCA23120.2187428154054737No Hit
GTATTTATTAGTGCCATCATATGATATAACTGCCACCCACTCCAGCCCCT22570.21353915846459953No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG21940.20757860596868913No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG21910.20729477013555053No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG21770.20597020291423712No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGTTATACT21720.20549714319233947No Hit
CTGCTATATTGACCCTCTCTCGCACAGTAATAAACAGCCGTGTCCTCAGG19990.18912927681468072No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT19430.18383100792942705No Hit
TCCTATGACAGTCTGAGTGTCCTATTCGGCGGAGGGACCAAGCTGACCGT18960.17938424654358914No Hit
GCCTGAGACCTGAGGACACGGCTGTTTATTACTGTGCGAGAGAGGGTCAA18520.1752213209908898No Hit
GTATAACTACTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGA18460.17465364932461264No Hit
GGGCTGGAGTGGGTGGCAGTTATATCATATGATGGCACTAATAAATACTA17810.16850387293994318No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC17730.16774697738490693No Hit
GTGCGAGAGAGGGTCAATATAGCAGCAGGTGGTTTGCGACTTCCAATTTT17250.16320560405468948No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC17090.161691812944617No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA16260.153839021561116No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA16130.1526090662841821No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG16040.15175755878476635No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG16040.15175755878476635No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC15770.14920303628651901No Hit
CTGCAGGACCGATTCACCATCTCCAGAGACAATTCCAAGAACACCGTGTA15700.14854075267586234No Hit
GGTCAATATAGCAGCAGGTGGTTTGCGACTTCCAATTTTGACCACTGGGG15690.14844614073148277No Hit
CTCTCGCACAGTAATAAACAGCCGTGTCCTCAGGTCTCAGGCTGTCCATT15310.14485088684506064No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA14950.14144485684739755No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT14940.14135024490301804No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG14720.13926878212666838No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG14700.1390795582379093No Hit
ATACTATCCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTG14480.13699809546155964No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGTTATACTATCCACTGGG14340.13567352824024623No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC14330.13557891629586669No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGT13910.13160521463192643No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC13820.13075370713251067No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGGACACTCAGACTG13590.12857763241178147No Hit
TTATTACTGTGCGAGAGAGGGTCAATATAGCAGCAGGTGGTTTGCGACTT13560.12829379657864287No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGGACTGGCT13560.12829379657864287No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGATTATGATGTACACT13530.12800996074550428No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT13470.1274422890792271No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC13460.12734767713484757No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13370.12649616963543178No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT13340.12621233380229321No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT13230.12517160241411837No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG13200.12488776658097978No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13090.12384703519280496No Hit
CTCCAGAGACAATTCCAAGAACACCGTGTATCTGCAAATGGACAGCCTGA13090.12384703519280496No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG13010.12309013963776871No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA12990.12290091574900966No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC12560.11883260214068986No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT12520.11845415436317172No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC12430.11760264686375597No Hit
GCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGTTCCACGACAC12410.1174134229749969No Hit
GTCCAGTGTCAGGTGCAGTTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC12350.11684575130871971No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT12170.11514273630988817No Hit
GTCTGGGGGAGGCGTGGTCCAGCCTGGGAGCTCCCTGAGACTCTCCTGTG12020.11372355714419523No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC11930.11287204964477945No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA11880.1123989899228818No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGGAC11820.11183131825660461No Hit
ATACTACTCAGAATCCCTGCAGGACCGATTCACCATCTCCAGAGACAATT11640.11012830325777309No Hit
CCCCAGGAGTTCAGGTGCTGGGCACGGTGGGCATGTGTGAGTTTTGTCAC11570.10946601964711637No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA11190.10587076576069424No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT11180.10577615381631471No Hit
TGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCT10920.1033162432624469No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC10780.10199167604113349No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG10770.10189706409675396No Hit
GGGCTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA10750.1017078402079949No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA10750.1017078402079949No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGGGCAGCCCCCAAACTCCTCAT10700.10123478048609724No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA10650.10076172076419959No Hit
GGTCTCAGGCTGTCCATTTGCAGATACACGGTGTTCTTGGAATTGTCTCT10580.10009943715354287No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)