FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1056949
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCCTCAGGTCTCAGGCTGTCCATTTGCAGATACA52580.4974696035475694No Hit
AATAAATACTACTCAGAATCCCTGCAGGACCGATTCACCATCTCCAGAGA51620.48838685688713457No Hit
GTTTATTACTGTGCGAGAGAGGGTCAATATAGCAGCAGGTGGTTTGCGAC44910.42490224220846984No Hit
ATATAGCAGCAGGTGGTTTGCGACTTCCAATTTTGACCACTGGGGCCAGG34140.3230051781117159No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33960.32130216311288434No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT29120.27550998203319177No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGTTGGTGGAGTCTGGGGGAGGCG28790.2723877878686673No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA27380.25904750371115354No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT27040.25583069760224947No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC26510.2508162645501344No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA24960.23615141317130722No Hit
GTAGTTATACTATCCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAG24770.23435378622809616No Hit
GTATTTATTAGTGCCATCATATGATATAACTGCCACCCACTCCAGCCCCT24680.23350227872868037No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC23370.22110811401496194No Hit
ATATTGACCCTCTCTCGCACAGTAATAAACAGCCGTGTCCTCAGGTCTCA21750.20578097902547804No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG21590.20426718791540557No Hit
TAGTTATACTATCCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGT21100.19963120264080858No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG20860.19736051597569987No Hit
GTATAACTACTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGA20670.1955628890324888No Hit
CTGCTATATTGACCCTCTCTCGCACAGTAATAAACAGCCGTGTCCTCAGG19860.18789932153774686No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGGCCAG19560.18506096320636095No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGTTATACT19470.1842094557069452No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA18980.1795734704323482No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT18260.17276141043702203No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG18250.1726667984926425No Hit
TCCTATGACAGTCTGAGTGTCCTATTCGGCGGAGGGACCAAGCTGACCGT18120.17143684321570862No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT16900.15989418600140592No Hit
GCCTGAGACCTGAGGACACGGCTGTTTATTACTGTGCGAGAGAGGGTCAA16700.15800194711381532No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC16300.15421746933863412No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC16140.15270367822856162No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG15860.1500545437859348No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15810.14958148406403715No Hit
GGGCTGGAGTGGGTGGCAGTTATATCATATGATGGCACTAATAAATACTA15560.1472161854545489No Hit
GTGCGAGAGAGGGTCAATATAGCAGCAGGTGGTTTGCGACTTCCAATTTT14830.1403095135148432No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGTTATACTATCCACTGGG14630.1384172746272526No Hit
CTCTCGCACAGTAATAAACAGCCGTGTCCTCAGGTCTCAGGCTGTCCATT14600.138133438794114No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG14460.13680887157280058No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC14370.13595736407338482No Hit
GGTCAATATAGCAGCAGGTGGTTTGCGACTTCCAATTTTGACCACTGGGG14170.1340651251857942No Hit
CTGCAGGACCGATTCACCATCTCCAGAGACAATTCCAAGAACACCGTGTA14070.1331190057419989No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA13970.1321728862982036No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA13740.1299968115774744No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCC13660.12923991602243817No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT13650.12914530407805863No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC13370.12649616963543178No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGATTATGATGTACACT13290.12573927408039554No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13210.12498237852535932No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG13210.12498237852535932No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT13140.1243200949147026No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC12900.12204940824959389No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG12820.12129251269455764No Hit
TTATTACTGTGCGAGAGAGGGTCAATATAGCAGCAGGTGGTTTGCGACTT12760.12072484102828046No Hit
ATACTATCCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTG12740.12053561713952139No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT12440.1176972588081355No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC12410.1174134229749969No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGT12230.11571040797616536No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT12180.1152373482542677No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG12150.11495351242112911No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG12030.11381816908857476No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA11850.11211515408974322No Hit
GTCTGGGGGAGGCGTGGTCCAGCCTGGGAGCTCCCTGAGACTCTCCTGTG11840.11202054214536367No Hit
GTCCAGTGTCAGGTGCAGTTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC11660.11031752714653215No Hit
CTCCAGAGACAATTCCAAGAACACCGTGTATCTGCAAATGGACAGCCTGA11660.11031752714653215No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC11470.10851990020332106No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11410.10795222853704389No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGGATAGTATAACTAC11360.10747916881514623No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC11340.10728994492638717No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA11210.10605998964945329No Hit
GTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGAC11020.1042623627062422No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT11000.10407313881748315No Hit
GCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGTTCCACGACAC10820.1023701238186516No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGGAC10700.10123478048609724No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG10670.10095094465295866No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10400.064.391251
TCAACGC10400.062.3141174
ATCAACG10750.060.2852943
CAACGCA11100.058.3844035
TATCAAC11350.057.0984042
AACGCAG12550.052.202686
CGCAGAG13750.047.123228
GGTATCA4400.044.1863751
GCAGAGT16350.039.189299
GTACATG20950.038.839331
TACATGG21250.038.2910082
ACATGGG21700.036.8332943
TATTGAG8800.036.821985
CGTACAG1102.53571E-432.7306485
TTATTGA6900.031.3075774
TTGAGGG6550.030.7759767
CATGGGG14900.030.4460964
ATTGAGG6700.030.0869646
ACGCAGA21650.029.5956047
GAATACG1952.7725582E-729.535929
Produced by FastQC (version 0.11.5)