FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1545341
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTTCAGGTCTCAGGCTGCTCATTTGCAAATACA62660.405476849446174No Hit
GTGTATTACTGTGCGAGAGGTTGGGGAAGCAGTAGTGTCACGAATTCTGG52900.34231926804504637No Hit
ATATTATGCAGCCTCCGTTGAGGACCGATTCTCCATCTCCAGAGACAATT41630.26939038050501474No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG33030.21373923295893915No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT32420.20979188412136868No Hit
ATCCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTG31660.20487387573357596No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT29570.19134935266714595No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC29500.19089637821037558No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA29210.18901976974661255No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA28750.18604308045926432No Hit
CCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGTC27840.18015441252124936No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT27150.175689378590227No Hit
GTCTCAGCCTCGCTCAATGTCCGGGTCTCCTGGACAGTCAGTCACCATCT26240.16980071065221203No Hit
CAGTTATATCATATGATGAAAGTACTAAATATTATGCAGCCTCCGTTGAG25870.16740641709499715No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC25810.16701815327490827No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC24800.16048237897007844No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG22420.14508124743988543No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG22250.14398116661630023No Hit
GCATAATATTTAGTACTTTCATCATATGATATAACTGACACCCACTCCAG21370.13828663058832968No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG20440.13226854137695174No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT19800.12812706062933682No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAGATTACGAAAATGGAG19470.1259916096188479No Hit
GTATTACTGTGCGAGAGGTTGGGGAAGCAGTAGTGTCACGAATTCTGGAG19220.1243738437018108No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT18910.12236781396468481No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT17700.11453782692622534No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA17590.11382600992272904No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG17130.1108493206353808No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC16930.10955510790175114No Hit
CAGTAATACACAGCCGTGTCTTCAGGTCTCAGGCTGCTCATTTGCAAATA16290.10541362715413621No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG16050.1038605718737806No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAGTCACTATCCTATGCACTGGG15720.10172512086329165No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG15510.10036619749298052No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23250.073.925551
GGTATCA11450.063.79711
ATCAACG27950.060.1372383
TCAACGC28400.059.4092674
CAACGCA29550.056.8552935
TATCAAC29900.056.7101942
AACGCAG30900.054.830516
CGCAGAG35550.047.4574978
GCAGAGT41900.040.2509169
ACGCAGA42900.039.1600277
TATCACG1052.01384E-434.0418477
CAGCGCT10200.032.2281579
GTAATAC32700.031.3180391
GTACATG37300.031.2957271
AATACAC35300.029.9877853
TACATGG37450.029.6117342
CAGCCGT34150.029.5057189
TAATACA37000.028.6183092
GTACACG16550.027.2146685
TACACAG42400.027.1469175
Produced by FastQC (version 0.11.5)