FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883293
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCTCAGATTTCAGGCTGCTCAACTCCATGTAGG30680.34733661423785767No Hit
GTCTATTACTGTGCGAGATTGATGGGCGGCTACAACGACCACGGTTTGGA28180.31903343511156546No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20590.23310498328414242No Hit
GTCTGGGGCCGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA18180.20582071860639675No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16430.1860084932179922No Hit
GCCTACATGGAGTTGAGCAGCCTGAAATCTGAGGACACGGCCGTCTATTA15810.17898930479467176No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15790.1787628793616614No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA15180.17185690365484613No Hit
TTCCAGGACAGAGTCACGATTACCGCGGACGAATCCACGAGCACAGCCTA14960.16936622389173242No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14920.16891337302571174No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13850.1567996123596587No Hit
GTCCTGGAAGCTCTGTGCGTAGTTTCTTGTATCAAAGATAGGGATGATCC13360.15125218925090542No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC12450.14094983204893508No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12160.13766666327028515No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG11720.13268530374405776No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11600.1313267511459957No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11490.13008141126443887No Hit
TCCCTATCTTTGATACAAGAAACTACGCACAGAGCTTCCAGGACAGAGTC11260.12747751878481997No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10880.12317543555762359No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT10540.11932620319644785No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA10410.11785443788188066No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10030.11355235465468423No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA9710.10992954772651885No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAGACCCGAGAGCCACTA9430.10675959166437411No Hit
CCATGTAGGCTGTGCTCGTGGATTCGTCCGCGGTAATCGTGACTCTGTCC9340.1057406772158276No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9130.10336321016921905No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATATT9100.10302357201970355No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC9080.1027971465866932No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9000.10189144485465186No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG8990.10177823213814668No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC9200.057.138464
ATCAACG9450.054.864843
CAACGCA10100.052.046915
AACGCAG10550.050.4951636
TATCAAC11500.045.084592
CGCAGAG12400.042.3810468
GGTATCA4150.039.9092141
ATAGACG13100.037.928994
AGACGGC13600.037.0535356
GTACATG19750.035.002381
AATAGAC15400.034.1346633
ACGCAGA15700.033.9314657
GCAGAGT15950.033.8509679
GTAATAG16100.032.6505471
TAATAGA16950.031.4380442
ACATGGG20400.031.4161763
GACGGCC17750.029.2014687
TTATACT4800.025.5035824
GTATCAA21500.025.4547351
CAGAGTA15000.025.436379
Produced by FastQC (version 0.11.5)