FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences952822
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT54990.5771277321472426No Hit
ACATTACCCCGTGGACGTTCGGCCCAGGGACCAAGGTGGAAGTCAAACGA44310.4650396401426499No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37780.39650637789639614No Hit
GGGTAATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGC31740.3331157341035367No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28830.3025748775741954No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG27670.29040051552126206No Hit
GTCCTGATCTATGCTGCATCCAGTTTGGAAAGTGGGGTCCCATCAAGGTT26770.280954889790538No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25890.27171916685383No Hit
GAGTTACATTACCCCGTGGACGTTCGGCCCAGGGACCAAGGTGGAAGTCA25540.2680458679585484No Hit
GATCTATGCTGCATCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCATTG24500.25713092266971166No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG24160.2535625751714381No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG22410.23519608069503015No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21180.22228705886304054No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT20330.21336619011735666No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20140.21137211357420377No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG18390.19300561909779582No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17870.18754814645337742No Hit
ATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGCAGACT17680.18555406991022458No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16820.17652824976753265No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA16640.17463912462138784No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA16450.17264504807823497No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16020.168132138006889No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT15470.16235981117144652No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15260.16015583183427753No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG14380.15092010889756954No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA14140.14840127536937645No Hit
ATGCTGCATCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCATTGGCAGT14100.14798146978134424No Hit
ACTTACTACTGTCAACAGAGTTACATTACCCCGTGGACGTTCGGCCCAGG14090.14787651838433621No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13750.14430817088606265No Hit
CACCATCAGCAGTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAAC13500.14168438596086153No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA13330.13990021221172474No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13120.1376962328745558No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA13110.13759128147754776No Hit
ATTTAAATTGGTATCAGCAGAAACCAGAGAAAGCCCCTAAGGTCCTGATC12700.13328827420021788No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTGGGCCGAACGTC12610.13234371162714548No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC12480.1309793434660409No Hit
GTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAACAGAGTTACATT12350.1296149753049363No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAGGATTTTGCAACTTA12110.1270961417767432No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC12100.12699119037973516No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12000.12594167640965467No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC11970.12562682221863053No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA11730.12310798869043746No Hit
GATCAGGACCTTAGGGGCTTTCTCTGGTTTCTGCTGATACCAATTTAAAT11520.1209040093532685No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11440.12006439817720414No Hit
CCCCTAAGGTCCTGATCTATGCTGCATCCAGTTTGGAAAGTGGGGTCCCA11220.11775546744302713No Hit
GTAGTAAGTTGCAAAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGA11210.11765051604601909No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAGGATT11160.11712575906097887No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG11080.11628614788491448No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11010.11555148810585819No Hit
GTCTCCGTCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT10970.115131682517826No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10580.11103857803451223No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC10570.11093362663750417No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT10540.11061877244648005No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGC10500.11019896685844786No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10390.10904450149135936No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC10380.1089395500943513No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCGGATGACCCAGTC10330.10841479310931107No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10280.10789003612427085No Hit
CCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTG10270.10778508472726281No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC10060.10558110539009384No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9860.10348207744993293No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCG9820.10306227186190076No Hit
ACTGTCAACAGAGTTACATTACCCCGTGGACGTTCGGCCCAGGGACCAAG9630.10106819531874789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23200.065.62581
ATCAACG23800.062.9690633
TCAACGC24200.061.9282534
CAACGCA24800.060.4268155
TATCAAC24600.060.3355032
AACGCAG25750.058.1455546
ACGCAGA28700.052.1634457
CGCAGAG29500.051.2368138
GCAGAGT32750.045.9324729
GAATACG5200.042.9084859
ATTACAC5100.035.3191833
GTGCTAG6350.035.2263871
GTGTAGA6500.034.413471
CTAGAGA6700.034.4124874
GTACATG27650.033.4037321
TACATGG28050.032.8789522
GCTAGAG7100.032.473753
TAAACCG900.003741131432.021055
CACCGGT900.003759450531.989127
ACCGTCG1204.2342197E-429.98988
Produced by FastQC (version 0.11.5)