FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences952822
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT49640.5209787347479382No Hit
ACATTACCCCGTGGACGTTCGGCCCAGGGACCAAGGTGGAAGTCAAACGA38700.40616190642113636No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34290.3598783403405883No Hit
GGGTAATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGC28300.297012453532769No Hit
GTCCTGATCTATGCTGCATCCAGTTTGGAAAGTGGGGTCCCATCAAGGTT27420.28777673059606096No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25790.2706696528837495No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG25380.26636664560641965No Hit
GATCTATGCTGCATCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCATTG24710.2593349020068806No Hit
GAGTTACATTACCCCGTGGACGTTCGGCCCAGGGACCAAGGTGGAAGTCA24140.25335267237742204No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23060.2420179215005531No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG21130.22176230187800028No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA20700.21724939180665434No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT19670.20643939791482563No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG18930.19867299453623027No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18830.19762348056614984No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18720.19646901519906132No Hit
ATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGCAGACT16640.17463912462138784No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16300.17107077712311428No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA16140.16939155477098555No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15750.1652984502876718No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15740.16519349889066373No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15520.16288456815648672No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT14730.15459340779285113No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14000.1469319558112638No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA13200.13853584405062017No Hit
ATGCTGCATCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCATTGGCAGT12960.13601701052242707No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTGGGCCGAACGTC12860.1349674965523466No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA12740.13370807978825006No Hit
ACTTACTACTGTCAACAGAGTTACATTACCCCGTGGACGTTCGGCCCAGG12680.1330783714062018No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG12670.13297342000919374No Hit
CACCATCAGCAGTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAAC12390.13003478089296847No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12330.1294050725109202No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC12170.12772585015879148No Hit
ATTTAAATTGGTATCAGCAGAAACCAGAGAAAGCCCCTAAGGTCCTGATC11740.12321294008744549No Hit
GATCAGGACCTTAGGGGCTTTCTCTGGTTTCTGCTGATACCAATTTAAAT11720.12300303729342942No Hit
GTCTCCGTCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT11430.11995944678019609No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA11350.11911983560413172No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC11320.1188049814131076No Hit
GTAGTAAGTTGCAAAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGA11260.11817527303105932No Hit
GTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAACAGAGTTACATT11210.11765051604601909No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAGGATTTTGCAACTTA11050.11597129369389036No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10900.11439702273876969No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10370.10883459869734326No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC10100.10600091097812603No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC9980.10474149421402948No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9940.1043216886259973No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGC9870.10358702884694097No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAGGATT9830.1031672232589088No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC9730.10211770928882835No Hit
CCCCTAAGGTCCTGATCTATGCTGCATCCAGTTTGGAAAGTGGGGTCCCA9680.1015929523037881No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)