FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621672
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT24420.3928116434389839No Hit
GTATTACTGTCAGCAGTATGCAAACTCACTGATCACCTTCGGCCAAGGGA23780.38251682559291716No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT16930.27233010333423413No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC14220.22873798401729528No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13980.22487742732502028No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13830.22246457939234837No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGCGT13250.21313490071935037No Hit
GTCTGGGACAGACTTCACGCTCACCATCAGCAGACTGGAGCCTGAAGATT12260.1972101043637159No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA11080.17822903396003037No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT10010.16101738537363755No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT9900.15924796355634482No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9390.1510442805852604No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9350.15040085446988122No Hit
GTGTATTACTGTCAGCAGTATGCAAACTCACTGATCACCTTCGGCCAAGG9090.1462185847199166No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCGCCAGCTACTTAGGCTGGTACC8970.1442883063737791No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGGT8780.14123203232572804No Hit
GTGTAGAGAACATATATGCATAAACATAGGTCAATTATATGTCTCCATTA8740.14058860621034885No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA8710.1401060366238145No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG8530.13721061910460822No Hit
TCCATGGTGCATCCACCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGC8460.13608462340269467No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8310.13367177547002276No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC8060.12965036224890295No Hit
ACTTAGGCTGGTACCAGCAGAAAACTGGCCAGGCTCCCAGGCTCCTCATC7880.12675494472969667No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7680.12353781415280084No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7600.12225096192204248No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7530.12112496622012894No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGCGTGA7450.11983811398937061No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA7070.11372556589326846No Hit
GTATATTACTGTGCCACTGGCGGATACTTCTCCTACGACTTCTGGGGCCA6950.11179528754713096No Hit
GTATGCAAACTCACTGATCACCTTCGGCCAAGGGACACGACTGGAGATTA6890.11083014837406222No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG6650.10696959168178719No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA6650.10696959168178719No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG6600.10616530903756322No Hit
GTAATATACGGCCGTGTCCGCGGCGGTCACAGAGCCCAGTTTCAGGGAGA6590.10600445250871843No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG9100.062.5079733
TCAACGC9250.061.4943354
GGTATCA3450.060.5241431
TATCAAC10150.056.751022
GTATCAA11050.054.7352031
CAACGCA10650.054.0866555
AACGCAG11200.051.4223376
CGCAGAG11800.048.1975488
GAATACG3300.047.9941839
ACGCAGA12200.046.6173027
GTGCTAG4350.046.3466641
GCAGAGT14100.041.3566939
GCTAGAG4650.037.162763
CTAGAGA5300.033.9636084
GTACATG15750.032.9155921
TACATGG15800.032.3557132
GGTATAC900.003751472832.001271
ACATGGG16100.031.3055923
TATACGG4600.029.7403095
ATACGGC4650.029.4157896
Produced by FastQC (version 0.11.5)