FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949190
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT25640.2701250539934049No Hit
GTATGATGATTTCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA20030.21102202930920044No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19800.20859891065013328No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17230.18152319345968668No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15590.16424530389068576No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC15350.1617168322464417No Hit
TCATCATACTGTAGACAGAAATATGTTGCAATATCTCCAGGCTGCAGGCT15030.15834553672078297No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA15000.15802947776525247No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14330.15097082775840454No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13750.1448603546181481No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13540.14264794192943459No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13060.1375909986409465No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12960.13653746878917813No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12880.13569464490776345No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG12420.13084840758962904No Hit
GTCTGGAGCAGAGGTGAAAAAGCCCGGGGAGTCTCTGAAGATCTCCTGTA11820.12452722847901894No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11780.12410581653831163No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11210.11810069638323202No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10940.11525616578345746No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG10740.11314910607992078No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10560.11125275234673773No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10540.11104204637638407No Hit
ATATATTTCTGTGCGAGACCTCGTAGAGATGGCTACAATTTGGGTTTAGA10340.10893498667284739No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA10280.10830286876178637No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG10260.1080921627914327No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9870.10398339636953613No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9800.10324592547329829No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9780.10303521950294461No Hit
GTCTCCATCCTCCCTTTCTGCTTCTGTGGGAGACAGAGTCACCATCACTT9640.10156027771046892No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT9570.10082280681423107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11650.054.990391
TCAACGC11350.053.205424
CAACGCA11500.053.144995
AACGCAG12150.051.496286
ATCAACG11950.050.531363
TATCAAC13700.044.601342
GAATACG2750.044.457929
CGCAGAG14150.043.2011578
GTACATG21250.037.9386441
TACATGG21900.036.107562
GTCTAAC1604.888352E-835.99091
ACGCAGA17150.035.6441047
CAGCGCT5900.035.349149
GCAGAGT18050.035.0621389
ACATGGG22000.034.63643
GTGCTAG3750.034.5512661
TACACGG8650.032.4251026
GTACACG8950.031.3316155
GCAGCGC6850.030.4467078
GTATGTA11050.029.9652751
Produced by FastQC (version 0.11.5)