FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923320
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTC21010.22754841225144043No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19790.21433522505740155No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA18390.1991725512281766No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT18170.1967898453407269No Hit
GCCTTGGGCTGACTCAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCGG18040.19538188277087032No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT17380.1882337651085214No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16980.18390157258588571No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTCATTATCACTGCAGCTCATAT16580.17956938006325002No Hit
GCTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAGGCCCCCAA15800.1711216046441104No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC15800.1711216046441104No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACTATGTCTCCT14490.15693367413247844No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14210.15390113936663347No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT13760.1490274227786683No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA13670.14805267946107525No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13470.14588658319975742No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13440.1455616687605597No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13130.14220421955551704No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG12630.1367889789022224No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA12550.13592254039769527No Hit
CATTATCACTGCAGCTCATATGCAGGCAGCAACAATTTACCGGTTTTCGG12380.1340813585755751No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12350.1337564441363774No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA12340.13364813932331154No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC12340.13364813932331154No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11810.12790798423081923No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT11780.12758306979162154No Hit
ATATATTACTGTGCGAGACCGACTACTCTGGTTCGGGGAGTTATAGAGGA11750.12725815535242385No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA11600.12563358315643547No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT10900.11805224624182298No Hit
TCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC10650.11534462591517568No Hit
GTGATAATGAGCCTCGTCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG10530.11404496815838497No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG10400.11263700558852836No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10350.11209548152319888No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10040.10873803231815622No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG10030.10862972750509033No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC9960.10787159381362907No Hit
GTAATATATGGCGGTGTCCGAGGCCTCCAGGCTGCTCCACTGCAAATAGG9850.10668024086990427No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATA9650.1045141446085864No Hit
GGTACCAACAGCACCCAGGCAAGGCCCCCAAACTCATGATTTATGAGGTC9350.10126500021660963No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG9310.10083178096434604No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)