FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812392
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG24050.2960393504613536No Hit
GATTATTACTGTGCGGCTTGGGACGTATCGGTCAATTGGGTGTTCGGCGG22480.27671370471398044No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC17040.2097509576657574No Hit
GTATATTACTGTGTGGCAGATCGGGGGTTTGGAGTGGTTATTTCAAACTA16470.20273464041989583No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGCTTCTCTGGGTCCAAG14530.17885454312696333No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14390.17713123713675172No Hit
GATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGCCGCTGCTGG14310.17614649085663078No Hit
GTAATAATCAGCCTCATCGTCGGACCGGAGCCCACTGATGGCCAGGGAGG13810.169991826605875No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC13550.166791401195482No Hit
ATATACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGGCTG12540.15435897940895527No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT11710.14414223675270066No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA11570.14241893076248904No Hit
ATATTACTGTGTGGCAGATCGGGGGTTTGGAGTGGTTATTTCAAACTACT11200.13786447921692976No Hit
GTCTGGGCCTGAGGTGAAGAAGCCTGGGACCTCAGTGAAGGTCTCCTGCA11200.13786447921692976No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAATTGACCGAT11100.1366335463667786No Hit
GTTCCTCACTATGGACTGGATTTGGAGGATCCTCTTCTTGGTGGGAGCAG10920.13441786723650653No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC10870.13380240081143094No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTATATTA10150.12493968429034259No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10110.12444731115028214No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9960.1226009118750554No Hit
CAGTAATATACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTA9710.1195235797496775No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9510.11706171404937517No Hit
GCCGTATATTACTGTGTGGCAGATCGGGGGTTTGGAGTGGTTATTTCAAA9440.11620006105426937No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9370.11533840805916355No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC9000.11078395651360427No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8810.10844518409831706No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT8800.10832209081330195No Hit
TAGTAGGACTGTTATCCAGTGGGTGCGACAGACTCGTGGACAACGCCTTG8690.10696806467813567No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA8690.10696806467813567No Hit
GTTATCCAGTGGGTGCGACAGACTCGTGGACAACGCCTTGAGTGGATGGG8570.10549094525795427No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8510.10475238554786359No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT8510.10475238554786359No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA8460.10413691912278802No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA8340.10265979970260662No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG8700.060.446423
TCAACGC8750.060.101014
CAACGCA9000.058.427945
GTATCAA9950.056.4900931
TATCAAC9750.054.6857722
TAGACCG553.322649E-452.3886835
AACGCAG10300.051.0283856
CGCAGAG10700.049.7936678
GGTATCA3900.044.3452841
GCAGAGT13900.038.8459639
GAATACG3650.037.4765979
ACGCAGA15250.034.93727
GTGCTAG4150.032.991821
TCTACAC7250.031.7964733
ATACGGC12500.031.1035486
TATACGG12650.030.7498825
GTACATG16350.030.4110981
ACATGGG16000.030.166283
GTAATAT13750.029.8725951
TACGGCC13200.029.4541177
Produced by FastQC (version 0.11.5)