FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences627887
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19400.3089727928751511No Hit
GTATAATAACTGGCCTCCGCCTTTCGGCCCTGGGACCAAAGTGGATATCA18250.29065739535935603No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17110.2725012621697853No Hit
GTAATACAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATACA16810.2677233323830562No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT13450.2142105187716898No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT11680.1860207330299879No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG11500.1831539751579504No Hit
ATACAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATACAGGG11270.17949089565479137No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10760.17136841501735184No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT10720.17073135771245462No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10510.16738680686174423No Hit
TTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCT10380.16531637062082827No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC9990.15910506189808038No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG9530.15177890289176238No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGC9390.1495492023246221No Hit
GCCTATGCTACCACTATTCCAACTAATACCTGAGACCCACTCCAGGCCCT8920.14206377899207978No Hit
TTGTATTACTGTGCAAAAGATAGAATTCCCGGGTGGGGGTTACCTAATCC8470.13489688431198607No Hit
AGCATAGGCTATGCGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA8420.13410056268086454No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT8170.13011895452525693No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7840.12486323175985488No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT7840.12486323175985488No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC7220.11498884353394799No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT6880.10957385644232162No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT6710.10686636289650846No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT6600.1051144553080411No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG6560.10447739800314387No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT6460.10288475474090084No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6290.10017726119508764No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14350.069.252441
GGTATCA5750.060.1147841
ATCAACG16350.059.445663
TCAACGC16500.058.9052474
CAACGCA16800.057.8533675
TATCAAC17450.055.698372
AACGCAG17750.054.7569926
ACGCAGA19400.050.0998277
CGCAGAG19650.049.462428
GAATACG1950.044.3048069
GCAGAGT22500.043.837149
GTGCTAG2900.032.281471
CCGTTGA4250.032.1861349
CCCGTTG4350.031.446228
ACCCCGT4400.031.088886
CCCCGTT4500.030.3980147
CTAGAGA3350.030.087594
CAGAGCG10050.029.3712189
ATAGGTG6650.029.2311744
TATAGGG1504.6106095E-528.7981222
Produced by FastQC (version 0.11.5)