FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences627887
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21100.3360477283332829No Hit
GTATAATAACTGGCCTCCGCCTTTCGGCCCTGGGACCAAAGTGGATATCA19920.31725453783881497No Hit
GTAATACAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATACA17180.27361611245335543No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15990.2546636576326632No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT13690.2180328626010731No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG11440.18219838920060458No Hit
ATACAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATACAGGG10760.17136841501735184No Hit
TTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCT10690.1702535647337817No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGC9980.1589457975718561No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT9880.15735315430961302No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC9770.1556012467211457No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT9600.15289375317533252No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9530.15177890289176238No Hit
GCCTATGCTACCACTATTCCAACTAATACCTGAGACCCACTCCAGGCCCT9330.14859361636727628No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG8830.14063040005606106No Hit
AGCATAGGCTATGCGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA8180.13027821885148125No Hit
TTGTATTACTGTGCAAAAGATAGAATTCCCGGGTGGGGGTTACCTAATCC7930.12629661069587364No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT7750.12342985282383614No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7600.12104088793047157No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG7360.11721854410108826No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT7150.11387399325037785No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC7130.11355546459792924No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT7080.11275914296680772No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT6980.11116649970456469No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6930.11037017807344314No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC6680.10638856991783553No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC6670.10622930559161121No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT6450.10272549041467653No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6440.10256622608845221No Hit
GCCTTGTATTACTGTGCAAAAGATAGAATTCCCGGGTGGGGGTTACCTAA6340.10097358282620918No Hit
CAGTAATACAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATA6300.10033652552131195No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG7450.057.0416263
TCAACGC7500.056.661354
GTACGGA1051.1768861E-954.8603326
GTATCAA8250.051.5267451
CAACGCA8600.049.4139675
GGTATCA3300.048.0334051
AACGCAG9150.047.2158556
AGTACGG1255.500624E-946.0973665
TATCAAC9650.045.5337372
ACGCAGA9950.043.4196057
CGCAGAG10400.041.5342568
ATAGGTG9400.040.6110464
GTCATAG10200.040.2632941
CCGTTGA3650.039.448069
TAGTACG1901.382432E-1037.909024
GCAGAGT11800.037.2165689
CCCGTTG3950.036.4520078
ATAGACC800.002102489136.0135653
CATAGGT10800.035.3466533
TAGGTGG11050.035.1987845
Produced by FastQC (version 0.11.5)