FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences560773
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGTATACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA25290.45098462301145026No Hit
GTATACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGAACT17890.3190239187692703No Hit
GTATACTATTGTGCGAGACATTGGTTTGGGGGAATTGTCGTCCAGCGCCG16950.3022613428249934No Hit
GTGTCTGGTGGCTCCGTCAGTGATAGGAGTTACTACTGGGGCTGGGTCCG15880.28318053829267814No Hit
CCATTATAGTGGGACCACCCACTACAAGCCGTCCCTCAAGAGTCGAGTCA15000.2674879140044189No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC13270.23663764125590928No Hit
GCTGTATACTATTGTGCGAGACATTGGTTTGGGGGAATTGTCGTCCAGCG12660.22575979941972954No Hit
CTCCATTATAGTGGGACCACCCACTACAAGCCGTCCCTCAAGAGTCGAGT10410.18563661231906672No Hit
TCATAAGACTGACAGTAGTAGTCAGCCTCGTCGTCAGTCTCCAGTCCAGA10190.18171345624700191No Hit
CAATAGTATACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA9920.17689867379492236No Hit
GTCTTATGATACCACCACTCAGGTGTTCGGCGGAGGGACCAAGCTGACCG9480.16905236165079274No Hit
ATACTATTGTGCGAGACATTGGTTTGGGGGAATTGTCGTCCAGCGCCGTA9470.1688740363747898No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTGAGTGGTGGTA9310.16602083195874268No Hit
CTACAAGCCGTCCCTCAAGAGTCGAGTCACCATGTCGATAGACACGTCCA8550.15246811098251878No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8410.14997155711847754No Hit
CTATAATGGAGACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCG8240.14694002742642745No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT8170.14569175049440683No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8040.14337352190636854No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT7920.14123361859433317No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT7870.14034199221431845No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7840.13980701638630963No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA7760.13838041417828603No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTG7720.13766711307427426No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA7620.13588386031424482No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA7410.13213902951818293No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC7410.13213902951818293No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC7310.13035577675815346No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7140.1273242470661034No Hit
TCTCTGGACTGGAGACTGACGACGAGGCTGACTACTACTGTCAGTCTTAT7110.12678927123809455No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG6860.12233113933802092No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA6850.12215281406201797No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAACTGTCGATGGAGCCA6700.11947793492197377No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG6680.11912128436996788No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT6660.11876463381796198No Hit
GTGTCGGAGTCTCCGGGGAAGGCGGTAACCATCTCCTGCACCCGCAGCAG6550.11680305578192958No Hit
CACGTGCACTGTGTCTGGTGGCTCCGTCAGTGATAGGAGTTACTACTGGG6420.1144848271938913No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC6380.1137715260898795No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG6250.1114532975018412No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6240.11127497222583825No Hit
GTAGTAGTCAGCCTCGTCGTCAGTCTCCAGTCCAGAGATGGTGAGGGAGG6200.11056167112182648No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA6170.11002669529381764No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA6170.11002669529381764No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG6110.10895674363779997No Hit
ATAATGGAGACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGA6010.10717349087777049No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC5980.10663851504976167No Hit
ACCGAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGT5900.10521191284173809No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC5900.10521191284173809No Hit
ACTATGTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCAATGTG5880.10485526228973222No Hit
GAGTTACTACTGGGGCTGGGTCCGCCAGCCCCCAGGGAAGGGGCTGGAGT5750.10253703370169391No Hit
GGTACCAGCAGCGCCCGGGCAGTGCCCCCACCAATGTGATTTATGAGGAT5740.10235870842569096No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAT350.003667298261.703626
GGACCAT1355.249967E-737.326886
TATTGAG8000.036.90355
TATTAGG2800.036.0130542
TTATTGA8550.035.374934
AGTATAC13050.034.765023
ATTGAGT5700.034.099376
GTATCAA13950.032.539531
CAGCCGT14250.032.3312539
ACAGCCG14300.032.218218
GACTCGG3800.032.204967
CAACGCA14350.032.1145445
AACGCAG14350.032.105956
CTAGTAC900.003748509632.005893
ATCAACG14250.031.8374373
TCAACGC14300.031.7261184
TATCAAC15000.030.731142
ATAGTAT14900.030.4648651
GTACTAG950.00487104830.3376141
ACGCAGA15350.030.0143557
Produced by FastQC (version 0.11.5)