FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577817
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA20960.3627446059911702No Hit
GTGTATTACTGTGCGAGAGGTCAAAATAAAGATAGCAGCTCGTCCTTCTG15300.26478971715958516No Hit
TACATATACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGA14530.2514636987142988No Hit
GTATATGTAACTACTACTACTACTAATGGATGAGACCCACTCCAGCCCCT11750.2033515801715768No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8640.14952831086658924No Hit
ATACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGACAACG8550.14797072429506228No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8490.14693233324737764No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA8260.14295183423125316No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7090.12270320880140252No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC7080.12253014362678841No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG6970.12062642670603323No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG6850.11854964461066393No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC6750.11681899286452285No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC6580.11387688489608301No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC6450.1116270376260996No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA6070.10505056099076353No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG6070.10505056099076353No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)