FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577817
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA20390.3528798910381661No Hit
GTGTATTACTGTGCGAGAGGTCAAAATAAAGATAGCAGCTCGTCCTTCTG14760.2554441977304233No Hit
TACATATACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGA14030.24281043998359342No Hit
GTATATGTAACTACTACTACTACTAATGGATGAGACCCACTCCAGCCCCT10800.18691038858323655No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9610.16631563280415773No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA8380.14502861632662245No Hit
ATACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGACAACG8220.14225957353279672No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8100.14018279143742743No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC7550.1306642068336515No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC7140.12356853467447307No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA6830.1182035142614357No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG6560.11353075454685481No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC6480.11214623314994195No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC6320.10937719035611622No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG6190.10712734308613281No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG6070.10505056099076353No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6550.057.1815221
ATCAACG6300.057.149223
TCAACGC6500.056.4985924
CAACGCA6550.054.967955
AACGCAG7400.049.622856
TATCAAC8050.044.7254752
TACGACG657.584127E-444.3126225
CGCAGAG8950.040.2244578
GGTATCA3500.039.100221
TACATGG12350.037.8989522
GTACATG13450.036.9505161
ACGACGG800.002105986736.000896
TATTGAG5000.034.5638475
CCGTACT850.002834071933.8861242
ACATGGG13600.033.8861243
GCAGAGT12250.029.3859379
TTATTGA6000.028.8032044
CAGCCGT12550.028.6834819
GTAATAC13600.027.5396291
AATACAC13800.027.1334533
Produced by FastQC (version 0.11.5)