FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653938
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATACA31410.4803207643538072No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21520.3290831852560946No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGGTTGGAGATGGTGAGGTAGC20300.31042698237447586No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG19080.2917707794928571No Hit
GTGTATTACTGTGCGAGAGATCTGTACGATATTTTGACTGGTTATTATAA16840.2575167676446391No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC16590.2536937752508648No Hit
CTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGAT15610.23870764506726938No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCGAGTGTTACTGT15490.2368726087182577No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCGAGTGTTACTG15460.23641384963100479No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA14020.21439341344286464No Hit
GATTATTACTGTGAGACCTGGGACAGTAACACTCGAGTCTTCGGAACTGG13550.20720618774256885No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC13530.20690034835106694No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG13410.20506531200205524No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13320.20368903474029648No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT13280.2030773559572926No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA13140.20093648021677896No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT12720.19451385299523807No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT11900.18197443794365828No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG11240.171881738024094No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC10980.16790582593456874No Hit
GTCCCAGGTCTCACAGTAATAATCAGCCTCATCCTCAGACTGGAGGTTGG10760.1645415926280473No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT9800.14986130183595386No Hit
TCCTCAGACTGGAGGTTGGAGATGGTGAGGTAGCGGTCAGCCCCAGAGCT9330.14267407613565813No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC9310.14236823674415616No Hit
GTACTTGATGAAGCTTGAAGGTAGTGGAAGCTACAACAAGGGGAGCGGAG9270.14175655796115227No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA9230.14114487917814839No Hit
CAGTAATAATCAGCCTCATCCTCAGACTGGAGGTTGGAGATGGTGAGGTA9080.1388510837418838No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC8980.13732188678437404No Hit
CAGTAGCTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTC8980.13732188678437404No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8920.1364043686098682No Hit
GTATGTGCTACCACCGCTATAAATAACTGAGACCCACTCCAGCCCCTTCC8890.13594560952261528No Hit
TTATTACTGTGAGACCTGGGACAGTAACACTCGAGTCTTCGGAACTGGGA8870.13563977013111334No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT8690.1328872156075958No Hit
AGCACATACTACGCAGACTCCGTGAAGGGCAGATTCACCATCTCCAGAGA8680.13273429591184485No Hit
GCTCTGGGGAGTCTGCACCATGGCCTGGACCCCACTCCTCCTCCTCTTCC8660.1324284565203429No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC8600.13151093834583705No Hit
AGCTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTCGGTA8520.1302875807798293No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8150.12462955203704326No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT7970.12187699751352575No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATA7860.12019488086026503No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA7840.1198890414687631No Hit
GTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAA7730.11820692481550239No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTTATAATAACCAG7700.11774816572824946No Hit
GTAGCTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTCGG7690.1175952460324985No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT7620.11652480816224167No Hit
ATAATAACCAGTCAAAATATCGTACAGATCTCTCGCACAGTAATACACAG7610.11637188846649071No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG7560.11560728998773584No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG7550.11545437029198487No Hit
ATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATACAGCG7460.1140780930302261No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCG7330.11209013698546344No Hit
ACCTGGGACAGTAACACTCGAGTCTTCGGAACTGGGACCAAGGTCACCGT7290.11147845820245955No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCT7270.11117261881095761No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT7260.11101969911520664No Hit
GTACATGGGAGCAGAGCTCTGGGGAGTCTGCACCATGGCCTGGACCCCAC7210.11025510063645176No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC7150.10933758246194593No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC7140.10918466276619496No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCCTGTGCTGACTCAATCAT7090.10842006428744008No Hit
CTCCTGTGCAGCCTCTGGATTCACCGTCAGTAGCAACTACATGAGCTGGG7090.10842006428744008No Hit
GTATCAACGCAGAGTACATGGGAGCAGAGCTCTGGGGAGTCTGCACCATG7040.10765546580868524No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG6900.1055145900681716No Hit
GAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGT6820.10429123250216381No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCGTCAGTAGCAACTAC6810.10413831280641284No Hit
GGCCTGGACCCCACTCCTCCTCCTCTTCCCTCTCCTCCTCCACTGCACAG6730.10291495524040505No Hit
ACTCTGAGCAGTGGGCACAGTAGCTACATCATCGCATGGCATCAGCAGCA6620.10123283858714435No Hit
ATACTACGCAGACTCCGTGAAGGGCAGATTCACCATCTCCAGAGACAATT6610.10107991889139337No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC6600.10092699919564241No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6350.061.2644921
GTATCAA13450.060.5264171
CCCTACG400.00621651854.0029532
CCGGTCG400.006220280753.9946949
TCAACGC15150.052.755364
ATCAACG15300.052.2381553
CAACGCA15500.051.5641145
TATCAAC15700.050.907242
AACGCAG16100.049.6424676
GTCATAG7650.048.028341
ATAGGTG7500.048.002624
TAGGTGG8150.046.8246465
CGCAGAG17900.044.643668
GTGGTCA8900.041.254378
GCAGAGT19950.040.0562179
AGGTGGT9500.038.6547476
GCACACA8200.038.636262
CTATATA1752.5956979E-937.0305944
CAGAGCG8400.036.8535239
GTCTTAC850.002827696733.9023551
Produced by FastQC (version 0.11.5)