FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653938
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATACA30870.47206310078325464No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG24230.3705244228046084No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGGTTGGAGATGGTGAGGTAGC19090.2919236991886081No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18110.2769375690050127No Hit
CTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGAT16260.2486474252910826No Hit
GTGTATTACTGTGCGAGAGATCTGTACGATATTTTGACTGGTTATTATAA15980.2443656738100554No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG15250.23320253602023433No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC15240.23304961632448334No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCGAGTGTTACTGT15180.2321320981499775No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCGAGTGTTACTG14890.2276974269731993No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT13860.21194669831084903No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA13750.21026458165758835No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA13540.20705326804681792No Hit
GATTATTACTGTGAGACCTGGGACAGTAACACTCGAGTCTTCGGAACTGG13300.20338319534879457No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT13030.19925436356351822No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT12800.19573721056124588No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC12200.18656202881618747No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT11800.18044524098614853No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC11420.17463429254761156No Hit
GTCCCAGGTCTCACAGTAATAATCAGCCTCATCCTCAGACTGGAGGTTGG10380.1587306441895103No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT10040.15353137453397722No Hit
GTACTTGATGAAGCTTGAAGGTAGTGGAAGCTACAACAAGGGGAGCGGAG9540.14588538974642856No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA9200.14068612009089548No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG9130.13961568222063864No Hit
CAGTAGCTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTC8930.13655728830561917No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC8840.1351810110438604No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC8790.13441641256510556No Hit
TTATTACTGTGAGACCTGGGACAGTAACACTCGAGTCTTCGGAACTGGGA8600.13151093834583705No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA8520.1302875807798293No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT8510.1301346610840783No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC8480.12967590199682538No Hit
AGCTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTCGGTA8360.1278408656478137No Hit
GTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAA8320.12722918686480983No Hit
CAGTAATAATCAGCCTCATCCTCAGACTGGAGGTTGGAGATGGTGAGGTA8220.12569998990730008No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC8110.12401787325403937No Hit
GTATGTGCTACCACCGCTATAAATAACTGAGACCCACTCCAGCCCCTTCC8090.12371203386253743No Hit
AGCACATACTACGCAGACTCCGTGAAGGGCAGATTCACCATCTCCAGAGA7990.1221828369050277No Hit
GCTCTGGGGAGTCTGCACCATGGCCTGGACCCCACTCCTCCTCCTCTTCC7990.1221828369050277No Hit
GTAGCTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTCGG7950.12157115812202379No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATA7850.12004196116451407No Hit
TCCTCAGACTGGAGGTTGGAGATGGTGAGGTAGCGGTCAGCCCCAGAGCT7830.11973612177301211No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCCTGTGCTGACTCAATCAT7790.11912444299000823No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG7780.11897152329425727No Hit
ATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATACAGCG7610.11637188846649071No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT7570.11576020968348683No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA7270.11117261881095761No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT7240.11071385972370468No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG7180.10979634154919884No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC7070.10811422489593815No Hit
CTCCTGTGCAGCCTCTGGATTCACCGTCAGTAGCAACTACATGAGCTGGG7060.10796130520018717No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC7040.10765546580868524No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7040.10765546580868524No Hit
GAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGT6990.10689086732993036No Hit
ATAATAACCAGTCAAAATATCGTACAGATCTCTCGCACAGTAATACACAG6940.10612626885117549No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCT6910.10566750976392257No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC6910.10566750976392257No Hit
GCACAGGGTCTCTCTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCT6900.1055145900681716No Hit
ACCTGGGACAGTAACACTCGAGTCTTCGGAACTGGGACCAAGGTCACCGT6780.10367955371915992No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTTATAATAACCAG6780.10367955371915992No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA6710.10260911584890312No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCGTCAGTAGCAACTAC6690.10230327645740116No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCG6690.10230327645740116No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT6630.10138575828289532No Hit
GATCTGCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGG6600.10092699919564241No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTTG3100.044.126948
TAGGTGG10050.042.999495
ATAGGTG10150.041.866254
GTCATAG10050.041.5757141
CCGTTGA3400.040.2303169
CAGAGCG7950.039.8441249
CCCCGTT3450.039.65037
ACCCCGT3450.039.65036
TCAACGC9800.037.481954
GTATCAA10300.037.0694731
ATCAACG9950.036.9168973
CAACGCA10050.036.5495645
TATCAAC10350.036.1860432
ATCTCAC4000.036.0203361
GTATGAC800.002102981836.012073
GTGGTCA11400.035.99838
AACGCAG10350.035.4765856
TCTACAC4400.034.3751563
GCACACA8900.033.9889222
ATGCAAC2353.6379788E-1133.721171
Produced by FastQC (version 0.11.5)