FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616749
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGTATACCACGCAGACTCCGTGAAGGACCGGTTCACCAGCTCCAGGGA21890.35492558561100224No Hit
GTAATAAACGGCCGTGTCCTCGGGTCTGAGGCTGTCCATTTCCAGATACA19960.32363246636800386No Hit
GTATACAATTGCACCACTCCCACTAATTCCTGAGACCCACTCCAGCCCCT18240.29574429792346646No Hit
GTAATAATCACCCTCATCTTCGGACCGGAGCCCACTGATGGCCAGGGAGG14270.231374513781133No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13740.22278106652787438No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12580.20397276687923288No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC11740.19035296368538904No Hit
GTTTATTACTGTGCGAGCGGGGCTCAGTGGCCGGCTGACTACTGGGGCCA11570.18759657494377777No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11360.1841916241453168No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11010.17851670614788187No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10620.17219322609359722No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10330.16749115118143684No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10100.16376191935455103No Hit
GATTATTACTGTGCGGCATGGGATGACAGCCTGAGTGGTCCGCTATTCGG10080.16343763832612618No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9030.14641288433382138No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC8460.13717087502371306No Hit
CCTTTAGCACTTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG8380.13587375091001364No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8340.13522518885316392No Hit
CAATTGCACCACTCCCACTAATTCCTGAGACCCACTCCAGCCCCTTCCCT8050.13052311394100355No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG7580.12290250977301949No Hit
GTGCAATTGTATACCACGCAGACTCCGTGAAGGACCGGTTCACCAGCTCC7440.12063254257404551No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7360.1193354184603461No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA6890.11171481429236205No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG6890.11171481429236205No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT6780.10993126863602534No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC6760.1096069876076005No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG6740.10928270657917563No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT6690.10847200400811352No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT6620.10733702040862651No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCGGACCACTCAGG6520.10571561526650226No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA6450.10458063166701526No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG6370.10328350755331585No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC6330.10263494549646614No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10400.065.81041
TCAACGC12300.055.6174934
ATCAACG12900.053.0306323
CAACGCA13000.052.6227045
TATCAAC13600.050.3011132
AACGCAG13650.050.1168636
CGCAGAG15450.044.267238
GTACTAG951.06726126E-437.9184571
GCAGAGT19100.036.1847159
CAGCGCT3000.035.996259
GGTATCA10850.034.5285151
TAAACGG9500.034.110015
AAACGGC9650.032.833586
ATAAACG9800.032.3310244
GTACATG14300.032.2439461
TTAAGAC1352.2424752E-532.004453
TCTATAC2051.2147211E-831.6141533
TACATGG13900.031.6015192
GTGTATA3400.029.6656171
TATAGGA1703.172523E-629.6511824
Produced by FastQC (version 0.11.5)