FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616749
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGTATACCACGCAGACTCCGTGAAGGACCGGTTCACCAGCTCCAGGGA22550.3656268595490224No Hit
GTAATAAACGGCCGTGTCCTCGGGTCTGAGGCTGTCCATTTCCAGATACA18630.3020677779777511No Hit
GTATACAATTGCACCACTCCCACTAATTCCTGAGACCCACTCCAGCCCCT18280.2963928599803161No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16040.26007338479673253No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12590.2041349073934453No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12220.19813570836758554No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC12180.1974871463107358No Hit
GTTTATTACTGTGCGAGCGGGGCTCAGTGGCCGGCTGACTACTGGGGCCA12050.19537931962597424No Hit
GATTATTACTGTGCGGCATGGGATGACAGCCTGAGTGGTCCGCTATTCGG11070.17948954923315644No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10620.17219322609359722No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9700.15727629878605398No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9690.15711415827184155No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9230.1496556946180699No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8780.14235937147851072No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC8700.1410622473648113No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8440.1368465939952882No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8040.13036097342679112No Hit
CCTTTAGCACTTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG7880.1277667251993923No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG7870.12760458468517988No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT7540.12225394771616979No Hit
GTGCAATTGTATACCACGCAGACTCCGTGAAGGACCGGTTCACCAGCTCC7440.12063254257404551No Hit
CAATTGCACCACTCCCACTAATTCCTGAGACCCACTCCAGCCCCTTCCCT7290.11820043486085911No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG6950.1126876573776366No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT6950.1126876573776366No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT6930.11236337634921176No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC6710.10879628503653836No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT6580.10668845835177682No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC6480.10506705320965255No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT6440.10441849115280286No Hit
GTAATAATCACCCTCATCTTCGGACCGGAGCCCACTGATGGCCAGGGAGG6420.10409421012437799No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT6260.10149996189697916No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCG502.076646E-457.616364
GTATCAA7650.056.4957961
TCAACGC7850.055.9649354
ATCAACG8100.053.352813
CAACGCA8350.051.751225
TATCAAC8800.048.2903562
AACGCAG9000.047.998056
CGCAGAG10800.039.328558
GCAGAGT12150.034.958719
GTACATG14950.033.7274251
ATCGTCG900.003754413731.9961058
TACATGG14750.031.2520872
ACATGGG15700.029.8198033
CAGCGCT3900.029.5348669
GATCGTC1306.749262E-427.6911837
CTAGAGA2354.577487E-827.58234
TATTGAG4750.027.2919585
ATAATAC3255.4569682E-1126.5943223
GCAGCGC4100.026.3382598
GTACACG5550.025.9533145
Produced by FastQC (version 0.11.5)