FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872232
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATAGCCGTGTCCTCAGGTCTCAGGCTGTTGATCTGCAGATAGA77690.890703390840969No Hit
ATATATTACTGTGCGACACGAAGGGGGGACGACTACCGTACTAATTCTGA74880.8584871914811656No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG30330.3477285859725394No Hit
ACCTATGCTATGCACTGGGTCCGCCAGACTCCAGGCAAGGGGCTGGATTG29940.34325729851690834No Hit
GCTATATATTACTGTGCGACACGAAGGGGGGACGACTACCGTACTAATTC29170.3344293720019444No Hit
ATATATAGCCGTGTCCTCAGGTCTCAGGCTGTTGATCTGCAGATAGAGCC28740.3294994909611204No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT25300.29006044263452846No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA24490.2807739225343716No Hit
GCTATGCACTGGGTCCGCCAGACTCCAGGCAAGGGGCTGGATTGGGTGGC23380.2680479505452678No Hit
ATATTACTGTGCGACACGAAGGGGGGACGACTACCGTACTAATTCTGAAT23300.2671307633748819No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22760.2609397499747773No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC22360.256353814122848No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC21660.24832842638197175No Hit
CAGTAATATATAGCCGTGTCCTCAGGTCTCAGGCTGTTGATCTGCAGATA20190.2314751121261316No Hit
CTTCTACTACTGGGGCCTGGGCACCCTGGTCACCGTCTCCTCAGCCTCCA19700.2258573407075182No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA19670.22551339551862346No Hit
TTACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC19490.2234497243852553No Hit
ATATAGCCGTGTCCTCAGGTCTCAGGCTGTTGATCTGCAGATAGAGCCTG19410.22253253721486946No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG19160.21966632730741364No Hit
ACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCT17430.19983215474781937No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA17250.1977684836144512No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17220.19742453842555652No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17120.1962780544625742No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCACTACCTATGCTATGCACTGGG16090.1844692696438562No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16050.18401067605866328No Hit
ATGCTATGCACTGGGTCCGCCAGACTCCAGGCAAGGGGCTGGATTGGGTG15820.18137376294380395No Hit
CGGCTATATATTACTGTGCGACACGAAGGGGGGACGACTACCGTACTAAT15280.1751827495436994No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15160.17380696878812057No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC14380.16486439387685844No Hit
CTACCTATGCTATGCACTGGGTCCGCCAGACTCCAGGCAAGGGGCTGGAT13810.1583294352878592No Hit
TAGTAGAAGTATTCAGAATTAGTACGGTAGTCGTCCCCCCTTCGTGTCGC13430.15397279622852636No Hit
GTAGTAGAAGTATTCAGAATTAGTACGGTAGTCGTCCCCCCTTCGTGTCG13220.15156517990626348No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA12980.1488136183951059No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT12270.1406735822579314No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA12220.14010034027644022No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCACTACCTATGCT12170.13952709829494905No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA12130.13906850470975612No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG12070.13838061433196674No Hit
GTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC12060.1382659659356685No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC11680.13390932687633567No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC11580.13276284291335333No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT11570.1326481945170551No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG11510.1319603041392657No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT11490.13173100734666923No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11440.13115776536517806No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT11310.12966733621330107No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG11280.12932339102440635No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC11240.12886479743921342No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11020.1263425327206523No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC10890.12485210356877527No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC10740.12313237762430179No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC10590.1214126516798283No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10360.11877573856496895No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACACTCAGGCTG10310.11820249658347778No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT10280.1178585513945831No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC10210.11705601262049546No Hit
GCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA10050.11522163827972375No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA10030.11499234148712727No Hit
GTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTG9960.11418980271303966No Hit
ATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATT9960.11418980271303966No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC9910.1136165607315485No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA9560.10960386686111034No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC9530.10925992167221565No Hit
GTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGA9430.10811343770923332No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA9430.10811343770923332No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT9410.10788414091663687No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTTTGATGTACATT9340.10708160214254923No Hit
TACTAATTCTGAATACTTCTACTACTGGGGCCTGGGCACCCTGGTCACCG9330.106966953746251No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9320.10685230534995276No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG9310.10673765695365454No Hit
GGGCTGGATTGGGTGGCAGTTGTTTCATATGATGAAAGCGAGAAATACTA9170.10513257940547927No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG9130.10467398582028635No Hit
ACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGTTG9060.10387144704619872No Hit
CGTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTG9050.10375679864990048No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG9010.10329820506470755No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT8970.10283961147951462No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)