FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872232
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATAGCCGTGTCCTCAGGTCTCAGGCTGTTGATCTGCAGATAGA77320.8864614001779344No Hit
ATATATTACTGTGCGACACGAAGGGGGGACGACTACCGTACTAATTCTGA70060.8032266644654176No Hit
ATATATAGCCGTGTCCTCAGGTCTCAGGCTGTTGATCTGCAGATAGAGCC29350.33649304313531264No Hit
ACCTATGCTATGCACTGGGTCCGCCAGACTCCAGGCAAGGGGCTGGATTG28220.32353777435361236No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG27650.3170028157646131No Hit
GCTATATATTACTGTGCGACACGAAGGGGGGACGACTACCGTACTAATTC26370.3023278210384393No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25640.29395848810866837No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA22870.2622008823340579No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22170.2541754945931816No Hit
ATATTACTGTGCGACACGAAGGGGGGACGACTACCGTACTAATTCTGAAT21350.24477432609672656No Hit
GCTATGCACTGGGTCCGCCAGACTCCAGGCAAGGGGCTGGATTGGGTGGC20240.23204835410762276No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC19900.22815030863348285No Hit
CTTCTACTACTGGGGCCTGGGCACCCTGGTCACCGTCTCCTCAGCCTCCA18900.21668546900365956No Hit
ATATAGCCGTGTCCTCAGGTCTCAGGCTGTTGATCTGCAGATAGAGCCTG18820.21576828183327373No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC18750.2149657430591861No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA18620.2134753139073091No Hit
CAGTAATATATAGCCGTGTCCTCAGGTCTCAGGCTGTTGATCTGCAGATA18290.2096919168294674No Hit
TTACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC18030.20671105852571336No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17320.19857102238853885No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG16750.19203606379953958No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA16020.1836667308697686No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15940.18274954369938273No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15460.17724642067706758No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA15180.17403626558071705No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14850.17025286850287538No Hit
ACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCT14650.16795990057691074No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCACTACCTATGCTATGCACTGGG14570.16704271340652485No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14470.16589622944354254No Hit
ATGCTATGCACTGGGTCCGCCAGACTCCAGGCAAGGGGCTGGATTGGGTG13980.16027845802492915No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC13980.16027845802492915No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT13680.15683900613598217No Hit
CGGCTATATATTACTGTGCGACACGAAGGGGGGACGACTACCGTACTAAT12970.14869896999880766No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12800.1467499472617377No Hit
TAGTAGAAGTATTCAGAATTAGTACGGTAGTCGTCCCCCCTTCGTGTCGC12680.14537416650615892No Hit
CTACCTATGCTATGCACTGGGTCCGCCAGACTCCAGGCAAGGGGCTGGAT12620.1446862761283695No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG12400.1421640114098084No Hit
GTAGTAGAAGTATTCAGAATTAGTACGGTAGTCGTCCCCCCTTCGTGTCG12390.14204936301351018No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA11780.13505581083931797No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11490.13173100734666923No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC11410.13081382017628337No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT11330.12989663300589752No Hit
GTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC11030.12645718111695053No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC10910.12508140036137175No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC10900.12496675196507351No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCACTACCTATGCT10650.1221005420576177No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA10560.1210687064909336No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA10170.11659741903530255No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT10050.11522163827972375No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT10010.11476304469453082No Hit
GCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA10000.11464839629823258No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTTTGATGTACATT9830.11269937356116262No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC9830.11269937356116262No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG9660.11075035082409267No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCC9640.11052105403149622No Hit
GTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTG9550.1094892184648121No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC9420.1079987893129351No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC9420.1079987893129351No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG9220.10570582138697043No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG9210.1055911729906722No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG9100.10433004063139166No Hit
GCATAGGTAGTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGA8960.1027249630832164No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT8790.10077594034614643No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC8780.1006612919498482No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10400.066.490711
ATCAACG12250.056.4492533
TCAACGC12250.055.861244
TATCAAC12600.054.881222
CAACGCA13250.052.1889345
AACGCAG14000.049.8789986
CGCAGAG15450.044.7318468
CTGTGCG27100.042.5035069
TATATTA27300.042.4801752
GAATACG2800.041.1373259
TACTGTG29300.040.2949187
ACTGTGC29850.039.793648
TTACTGT29550.039.7103926
GTAATAT32500.038.3429761
ATATTAC30350.037.9738273
TAATATA32550.037.841492
TATATAG32850.037.4959035
TAGCCGT33400.036.426239
AGTGGTG7150.036.267665
GCAGAGT19300.036.181669
Produced by FastQC (version 0.11.5)